DEVELOPMENT OF HALOPLEX PANEL AND PREPARATION OF DNA LIBRARIES FOR TARGETED SEQUENCING OF CARDIAC ARRHYTHMIAS
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Method of preparation of custom HaloPlex cardiogenetic panel (Agilent Technologies) and method of preparation of DNA libraries for targeted sequencing of 96 genes associated with cardiac arrhythmias were described in details in this article. SureDesign Online Design software (Agilent Technologies) was used to create the HaloPlex panel. Final design was developed for Illumina platform using Human Genome version 19, GRCh 37. 19958 amplicons were generated by the program, 99,46% of all target regions were covered successfully. DNA libraries were prepared for 48 samples using the custom HaloPlex cardiogenetic panel.
Ainur Akhmetova1, Zhannur Abilova1, Makhabbat Bekbosynova2, Katrin Panzitt3, Slave Trajanoski3, Christian Guelly3, Ainur Akilzhanova1 1 National Laboratory Astana, Nazarbayev University, Astana, Kazakhstan; 2 National Research Cardiac Surgery Center, Astana, Kazakhstan; 3 Center for Medical Research, Medical University of Graz, Graz, Austria
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Ахметова А.Ж., Абилова Ж.М., Бекбосынова М.С., Panzitt K., Trajanoski S., Guelly C., Акильжанова А.Р. Разработка HaloPlex панели и подготовка ДНК-библиотек для таргетного секвенирования сердечных аритмий / / Наука и Здравоохранение. 2016. №5. С. 43-52. Akhmetova A., Abilova Zh., Bekbosynova M., Panzitt K., Trajanoski S., Guelly C., Akilzhanova A. Development of HaloPlex panel and preparation of DNA libraries for targeted sequencing of cardiac arrhythmias. Nauka i Zdravookhranenie [Science & Healthcare]. 2016, 5, pp. 43-52. Ахметова А.Ж., Абилова Ж.М., Бекбосынова М.С., Panzitt K., Trajanoski S., Guelly C., Акильжанова А.Р. HaloPlex панелін әзірлеу және жүрек аритмияларын таргетті секвенирлеуге ДНҚ-кітапханаларын дайындау / / Ғылым және Денсаулық сақтау. 2016. №5. Б. 43-52.

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