DIAGNOSTIC ROLE OF BIOCHEMICAL MARKERS OF BONE METABOLISM AND FDPS GENE IN THE EVALUATION OF SECONDARY OSTEOPOROSIS IN PATIENTS WITH CHRONIC PANCREATITIS AND HYPERTENSIVE DISEASE
Introduction. The development of a number of internal diseases occurs with the participation of calcium ions. Chronic pancreatitis (CP) and hypertensive disease (HD) are consideredamong such nosologies. The combined course of these diseases can lead to its competitive consumption, thus providing a way out of the repository-bone tissue. So, the conditions for the development of osteoporotic conditions are created.
Study purpose. Determination of the content of biochemical markers of bone metabolism and determination of their dependence on the polymorphism of the FDPS receptor gene c.IVS1 T-99G in patients with combined HD and CP.
Materials and methods. 110 patients were examined - 70 persons with HD and CP (main group) and a comparison group - 40 patients with isolated CP. Control group - 78 practically healthy persons. All patients were representative by age and sex. The content of biochemical markers of bone metabolism (total and ionized serum calcium, osteocalcin) and polymorphism of the FDPS gene. The statistical processing of the results was carried out using the STATISTICA software package. When analyzing the conjugacy tables, the Pearson criterion χ-square (QCP) was determined; for comparison of unbound samples of continuous scale indicators, the nonparametric Mann-Whitney criterion (CMC) was used.
Results. The course of CPis accompanied by quantitative changes in the indices of both forms of calcium without changing their relationship between the cell and the intercellular space. With the addition of HD, quantitative changes are saved against the background of a more significant increase in its free fraction within the cell. Changes in the level of osteocalcin in serum were also observed. When studying the polymorphism of the FDPS gene, there was an increase in the incidence of the CC genotype: 2.6 ± 1.8% - in the control; 22.5 ± 6.6% and 30.0 ± 5.5% in HP and HP + HD, respectively, on the background of a decrease in individuals with the AA genotype (66.7 ± 5.3%, 40.0 ± 7.7 %, 47.1 ± 6.0%, respectively). Values of calcium and osteocalcin fractions did not have a statistically significant dependence on the polymorphism of the FDPS gene.
Conclusions. In HP, conditions are created for the formation of a negative balance of calcium metabolism, which are enhanced by the addition of HD. The combined course of HP and HD is accompanied by changes in the content of osteocalcin, the level of which correlated with a violation of bone mineral density. An increase in individuals with the C-allele of the FDPS gene was noted, but this polymorphism of the gene had no clinical confirmation.
Changes in the rates of calcium metabolism and osteocalcin content indicate a violation of bone mineral density with the development of osteoporotic conditions.
Tatiana I. Viun 1, http://orcid.org/0000-0002-7862-349X
Lyudmila M. Pasiyeshvili 2, https://orcid.org/0000-0001-7527-782X
Department of General Practice - Family and Internal Medicine,
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19. Lanas A. Assessment of gastrointestinal and cardiovascular risk in patients with osteoarthritis who require NSAIDs: the LOGICA study / A. Lanas, J. Tornero, J.L. Zamorano. Ann. Rheum. Dis. 2009. V. 68. S. 11. P. 1696-1700.
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22. Liu Y., Liu H., Li M., et al. Association of farnesyl diphosphate synthase polymorphisms and response to alendronate treatment in Chinese postmenopausal women with osteoporosis. Chin Med J (Engl). 2014; 127(4):14-21, 662-8. doi: 10.3760/cma.j.issn.0366- 6999.20132382
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24. Marini F., Falchetti A., Silvestri S., et al. Modulatory effect of farnesyl pyrophosphate synthase (FDPS) rs2297480 polymorphism on the response to long-term amino-bisphosphonate treatment in postmenopausal osteoporosis. CurrMed Res Opin. 2008 Sep. 24(9): 2609-15. doi: 10.1185/03007990802352894
25. Olmos J.M., Zarrabeitia M.T., Hernandez J.L., et al. Common allelic variants of the farnesyl diphosphate syntase gene influence the response of osteoporotic women to bisphosphonates. Pharmacohenom J. 2012; 12:227-32.doi: 10.1038/tpj.2010.88
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28. Shimokawa H., Satoh K. Vascular function. Arterioscler. Thromb. Vasc. Biol. 2014. Vol. 34, No. 11. P. 2359–2362.
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