MOLECULAR CHARACTERIZATION OF MULTIDRUG RESISTANT M.TUBERCULOSIS STRAINS DISTRIBUTED IN THE TERRITORY OF KAZAKHSTAN
In spite of that the rate of tuberculosis is decreasing every year in Kazakhstan wide distribution of drug-resistant tuberculosis threatens tuberculosis control. According to various studies, M.tuberculosis W-Beijing genotype is associated with drug resistance and more severe forms of tuberculosis. Aim of the study: estimation of mutations spectrum in katG, fabG-inhA, oxyR-ahpC and rpoB responsible for drug resistance of M. tuberculosis to isoniazid and rifampicin, and determination of genetic families of 103 multidrug resistant clinical isolates of M.tuberculosis spread in Kazakhstan by Sanger method and spoligotyping, respectively. Among 103 multidrug resistant strains in Kazakhstan mutations at 531 codon Ser→Leu of rpoB gene (87,4%) and at 315 codon Ser→Thr of katG gene (97%) responsible for resistance to rifampicin and izoniazid respectively were prevailed. More than 80% of multidrug resistant M.tuberculosis strains were referred to Beijing genotype, the most virulent and widely spread genotype in the world. M. tuberculosis Beijing family strains prevail among multidrug and drug resistant strains in Kazakhstan.
Ainur Zh. Аkhmetova1,2, http:// orcid.org/0000-0002-5557-3338
Ainur R. Akilzhanova1,2, http://orcid.org/0000-0001-6161-8355
Askhat B. Molkenov1, http://orcid.org/0000-0003-2339-5204
Ulykbek E. Kairov1, http://orcid.org/0000-0001-8511-8064
Venera L. Bismilda3, SCOPUS ID: 8441834800
Lyailya T. Chingissova3, SCOPUS ID: 39261046300
Ulan A. Kozhamkulov1, http://orcid.org/0000-0002-9782-7631
1 National Laboratory Astana, Nazarbayev University, Nur-Sultan city, Republic of Kazakhstan;
2 L.N.Gumilyov Eurasian National University, Nur-Sultan city, Republic of Kazakhstan;
3 National Scientific Center for Phthisiopulmonology of the Republic of Kazakhstan,
Almaty city, Republic of Kazakhstan.
1. Afanas’ev M., Ikryannikova L., Il’ina E., Kuz’min A., Larionova E., Smirnova T., Chernousova L., Govorun V. Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation // Eur J Clin Microbiol Infect Dis. 2011. 30 (2): 181-91.
2. Ahmad S., Mokaddas E. Recent advances in the diagnosis and treatment of multidrug-resistant tuberculosis // Respir Med. 2009. №103. P. 1777–1790. 10.1016/j.rmed.2009.07.010.
3. Akhmetova A., Kozhamkulov U., Bismilda V., Chingissova L., Abildaev T., Dymova M., Filipenko M., Ramanculov E. Mutations in the pncA and rpsA genes among 77 Mycobacterium tuberculosis isolates in Kazakhstan // Int J Tuber Lung Dis. 2015. 19 (2): 179-184.
4. Chin K.L., Sarmiento M.E., Norazmi M.N., Acosta A. DNA markers for tuberculosis diagnosis // Tuberculosis. 2018. №113. P.139-152.
5. Demay C., Liens B., Burguiere T., Hill V., Couvin D., Millet J., Mokrousov I., Sola C., Zozio T., Rastogi N. SITVITWEB – A publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology // Infection, Genetics and Evolution. 2012. №12. P. 755-766.
6. European Concerted Action on New Generation Genetic Markers and Techniques for the Epidemiology and Control of Tuberculosis. Beijing/W genotype Mycobacterium tuberculosis and drug resistance // Emerg Infect Dis. 2006. №12. P. 736-743.
7. Jagielski T., Bakuła Z., Roeske K., Kamiński M., Napiórkowska A., Augustynowicz-Kopeć E., Zwolska Z., Bielecki J. Detection of mutations associated with isoniazid resistance in multidrug-resistant Mycobacterium tuberculosis clinical isolates // J Antimicrob Chemother. 2014. №69 (9). P. 2369-75. doi: 10.1093/jac/dku161. Epub 2014 May 22.
8. Hillemann D., Kubica T., Agzamova R., Bismilda V., Rusch-Gerdes S., Niemann S. Rifampicin and isoniazid resistance mutations in Mycobacterium tuberculosis strains isolated from patients in Kazakhstan // Int J Tuber Lung Dis. 2005. 9 (10): 1161-1167.
9. Kozhamkulov U., Akhmetova A., Rakhimova S., Belova E., Alenova A., Bismilda V., Chingissova L., Ismailov S., Ramanculov E., Momynaliev K. Molecular characterization of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains isolated in Kazakhstan // Jpn J Infect Dis. 2011. 64 (253-255).
10. Kubica T., Agzamova R., Wright A., Aziz M.A., Rakishev G., Bismilda V., Richter E., Rusch-Gerdes S., Niemann. The Beijing genotype is a major cause of drug-resistant tuberculosis in Kazakhstan // Int J Tuberc Lung Dis. 2005. №9 (6). P. 646-653.
11. Liu L., Jiang F., Chen L., Zhao B., Dong J., Sun L., Zhu Y., Liu B., Zhou Y., Yang J., Zhao Y., Jin Q., Zhang X. The impact of combined gene mutations in inhA and ahpC genes on high levels of isoniazid resistance amongst katG non-315 in multidrug-resistant tuberculosis isolates from China // Emerg Microbes Infect. 2018. №7 (1). P. 183. doi: 10.1038/s41426-018-0184-0.
12. Luo D., Chen Q., Xiong G., Peng Y., Liu T., Chen X., Zeng L., Chen K. Prevalence and molecular characterization of multidrug-resistant M.tuberculosis in Jiangxi province, China // Sci Rep. 2019. 9 (1): 7315.
13. Mathuria J.P., Srivastava G.N., Sharma P., Mathuria B.L., Ojha S., Katoch V.M. et al. Prevalence of Mycobacterium tuberculosis Beijing genotype and its association with drug resistance in North India // J Infect Public Health. 2016.
14. Ministry of Health of the Republic of Kazakhstan. Statistic TB Review (Editor Adenov M.M.). National TB Center, Almaty, 2018. P. 75 (in Russian).
15. Miotto P., Zhang Y., Cirillo D.M, Yam W.C. Drug resistance mechanisms and drug susceptibility testing for tuberculosis // Respirology. 2018. №23. P. 1098-1113.
16. Myo T. Zaw, Nor A. Emran, Zaw Lin. Mutations inside rifampicin-resistance in Mycobacterium tuberculosis // Journal of Infection and Public Health. 2018. №11. P. 605-610.
17. Nguyen L. Antibiotic resistance mechanisms in M. tuberculosis an update // Arch. Toxicol. 2016. №90. P. 1585-604.
18. Prim R.I., Marcos M.A., Senna S.G., Nogueira C.L., Figueiredo A.C.C. and de Oliveira J.G. Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis // Mem Inst Oswaldo Cruz. 2015. №110. P. 618-623.
19. Ramazanzadeh R., Sayhemiri K. Prevalence of Beijing family in Mycobacterium tuberculosis in world population: systematic review and meta-analysis // Int J Mycobacteriol. 2014. №3 (1). P. 41-45.
20. Rattan A., Kalia A., Ahmad N. Multidrug-resistant Mycobacterium tuberculosis: molecular perspectives // Emerg Infect Dis. 1998. №4. P. 195–209.
21. Skiba Y., Mokrousov I., Ismagulova G., Maltseva E., Yurkevich N., Bismilda V., Chingissova L., Abildaev T., Aitkhozhina N. Molecular snapshot of Mycobacterium tuberculosis population in Kazakhstan: a country-wide study // Tuberculosis. 2015. №95. P. 538-546.
22. Sreevatsan S., Pan X., Zhang Y., Deretic V., Musser J.M. Analysis of the oxyR-ahpC region in isoniazid-resistant and-susceptible Mycobacterium tuberculosis complex organisms recovered from diseased humans and animals in diverse localities // Antimicrob Agents Chemother. 1997. №41. P.600–606.
23. Zhang Y., Heym B., Allen B., Young D., Cole S. The catalase-peroxidase gene and isoniazid resistance of Mycobacterium tuberculosis // Nature. 1992. №358. P. 591-3.
24. Zhang Y., Yew W.W. Mechanisms of drug resistance in Mycobacterium tuberculosis // Int J Tuberc Lung Dis. 2009. №13. P. 1320–1330.
25. World Health Organization (1998): Laboratory services in tuberculosis control. Part III: culture. Global Tuberculosis Programme (WHO/TB/98.258). Geneva: World Health Organization, 1998, P.10-28.
26. World Health Organization (2003): Guidelines for surveillance of drug resistance in tuberculosis: Second edition World Health Organization. Global Tuberculosis Programme (WHO/CDS/TB/2003.320). Geneva: World Health Organization, 2003, P.8-12.
27. World Health Organization. Global tuberculosis report 2017. Geneva: World Health Organization, 2017. P.21
28. World Health Organization. Global tuberculosis report 2018. Geneva: World Health Organization, 2018. P.1-4.
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Аkhmetova A.Zh., Akilzhanova A.R., Molkenov A.B., Kairov U.E., Bismilda V.L., Chingissova L.T., Kozhamkulov U.A. Molecular characterization of multidrug resistant M.TUBERCULOSIS strains distributed in the territory of Kazakhstan // Nauka i Zdravookhranenie [Science & Healthcare]. 2019, (Vol.21) 5, pp. 45-52.Related publications:
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