POLYMORPHISM OF IL10 GENE AT -1082G/A POSITION IN PATIENTS WITH ERYSIPELAS
The aim of this study is reveal the association of polymorphism of IL10 gene (rs1800896) at 1082 GA position with the erysipelas in Kazakh population.
Materials and methods. The polymorphism of IL10 gene (rs1800896) was assessed in 206 patients with erysipelas and 422 control subjects. Of them 143 patients with primary erysipelas and 63 with recurrence erysipelas. Genomic DNA was extracted from blood for genotyping. Genotyping of polymorphism of IL10 gene (-1082 GA) was conducted by method of polymerase chain reaction in real time. Pearson’s Chi tests and odds ratios (OR) with 95 % confidence intervals (CI) were using for statistical analysis.
Our results showed that G allele and GG genotype more common in both group: in patients and in control group (OR = 0.87; 95% CI: 0.68–1.12 and OR = 0.86; 95% CI: 0.61–1.19, respectively). Also statistical significant differences by the frequency of occurrence of allele A (OR = 1.14; 95% CI: 0.89–1.47) and AA (OR = 1.20; 95% CI: 0.73–2.00) genotype in patients with the control group are not revealed. When comparing patients with primary erysipelas (case group) and control group, and patients with recurrent erysipelas (case group) with a individuals of control group were also observed no statistically significant results.
Conclusions. So, our results suggested that no association of polymorphism of IL10 gene (rs1800896) at position -1082 GA with erysipelas in Kazakh population.
Nazira B. Bekenova 1, http://orcid.org/0000-0002-3312-3299
Andrej M. Grjibovski 2-5, http://orcid.org/0000-0002-5464-0498
Lidiya. A. Mukovozova 1, http://orcid.org/0000-0001-9732-3102
Nurlan Ye. Aukenov 1, http://orcid.org/0000-0002-3163-2997
1 Semey State Medical University, Semey, Kazakhstan;
2 Norwegian Institute of Public Health, Oslo, Norway;
3 Northern State Medical University, Arkhangelsk, Russia;
4 International Kazakh-Turkish University, Turkestan, Kazakhstan;
5 North-Eastern Federal University, Yakutsk, Russia.
1. Бедарева Т.Ю. Изменения цитокино-вого статуса в остром периоде клещевых нейроинфекций у детей // Бюллетень сибирской медицины. 2009. №1. С.10-14.
2. Бекенова Н.Б., Гржибовский А.М., Муковозова Л.А., Смаил Е. М., Токаева А.З., . Полиморфизм rs8193036 гена ИЛ-17А в казахской популяции и его связь с продукцией ИЛ-17А у больных рожей // Экология человека. 2016. №. 4. С.50-55.
3. Гржибовский А.М.. Иванов С.В., Горбатова М. А. Исследования типа «случай-контроль» в здравоохранении // Наука и Здравоохранение. 2015. № 4. С. 5-17.
4. Емельянова А.Н., Витковский Ю.А., Кижло Л.Б. , Калинина Э.Н. Прогностическое значение генетического полиморфизма молекул Il-2 (Т330G), Il-10 (С819Т), Il-10 (G1082A) у больных рожей в Забайкальском крае // Дальневосточный журнал инфекцион-ной патологии. 2012. № 21. С. 159-163.
5. Иванов В.В., Шипилов М.В. Интерлей-кин-10 и острые респираторные вирусные инфекции // Здоровье - основа человеческого потенциала-проблемы и пути их решения. 2011. №. 1. С.101-106.
6. Костина Е.М., Молотилов Б.А., Баранова Н.И., Левашова О.А. Особенности полиморфизма генов цитокинов ИЛ-4, ИЛ-10, ИЛ-17 А и ФНО – α у больных с различными клинико-патогенетическими вариантами инфекционно-зависимой бронхиальной астмы // Аллергология и иммунология. 2013. №1. С. 5-9.
7. Ризванова Ф. Ф. и др. Генетическая диагностика: полиморфизм генов цитокинов // Практическая медицина. 2010. №. 6. С.41-43.
8. Юрко Е.В. Характеристика цитокинового обмена у пациентов c ко-инфекцией ВИЧ/ХГС // Міжнародний медичний журнал. 2014. №. 20. С.72-74.
9. Alvarado-Arnez L. E. Amaral E.P., Sales-Marques C., Durães S. M., Cardoso C.C., Nunes Sarno E., Pacheco A.G., Lana F.C., Moraes M.O. Association of IL10 Polymorphisms and Leprosy: A Meta-Analysis //PloS one. 2015. Vol. 10(9). Р.1-13.
10. Brooks D. G, Trifilo M. ., Edelmann K.,Teyton L, Mc. Gavern D.B, Oldstone M.B. Interleukin-10 determines viral clearance or persistence in vivo // Nature medicine. 2006. Vol. 12(11). Р. 1301-1309.
11. da Silva N. M, Germano F.N, Vidalez –Braz B.M, Carmo Zanella Rd, dos Santos D.M, Lobato R, de Martinez A.M. Polymorphisms of IL-10 gene in patients infected with HCV under antiviral treatment in southern Brazil //Cytokine. 2015. Vol. 73(2). Р. 253-257.
12. Gao Q. J., Liu D.W., Zhang S.Y, Jia M, Wang L.M, Wu L.Y, Wang S.Y, Tong L.X. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection //World J Gastroenterol. 2009. Vol. 15(44). Р. 5610-5619.
13. McGuire W., Hill A.V., Allsopp C.E., Greenwood B.M, Kwiatkowski D. Variation in the TNF-α promoter region associated with susceptibility to cerebral malaria// Nature. 1994. Vol. 371(6497). P. 508-511.
14. Meenakshi P., Ramya S, Shruthi T, Lavanya J, Mohammed H.H., Vijayalakshmi V, Sumanlatha G. Association of IL‐1β+ 3954 C/T and IL‐10‐1082 G/A Cytokine Gene Polymorphisms with Susceptibility to Tuberculosis // Scandinavian journal of immunology. 2013. Vol. 78(1). Р. 92-97.
15. Mira J. P, Cariou A, Grall F, Delclaux C, Losser M.R, Heshmati F, Cheval C, Monchi M, Teboul J.L., Riché F., Leleu G., Arbibe L., Mignon A., Delpech M., Dhainaut J.F. Association of TNF2, a TNF-α promoter polymorphism, with septic shock susceptibility and mortality: a multicenter study // Jama. 1999. Vol. 282(6). Р. 561-568.
16. Mosaad Y. M., Soliman O. E., Tawhid Z. E., Sherif D. M. Interferon‐gamma+ 874 T/A and Interleukin‐10‐1082 A/G Single nucleotide Polymorphism in Egyptian Children with Tuberculosis // Scandinavian journal of immunology. 2010. Vol. 72 (4). P. 358-364.
17. Nadel S., Newport M. J., Booy R., Levin M. Variation in the tumor necrosis factor-α gene promoter region may be associated with death from meningococcal disease // Journal of Infectious Diseases. 1996. Vol. 174(4). Р. 878-880.
18. Pereira V. A., Sánchez-Arcila J.C., Teva A., Perce-da-Silva D.S., Vasconcelos M. P., Lima C. A., Aprígio C. J., Rodrigues-da-Silva R. N., Santos D.O., Banic D. M., Bonecini-Almeida M. G., Lima-Júnior J. C., Oliveira-Ferreira J. IL10A genotypic association with decreased IL-10 circulating levels in malaria infected individuals from endemic area of the Brazilian Amazon // Malaria journal. 2015. Vol. 28(14). Р. 1-12.
19. Rattanasiri S., Mc Daniel D.O., Mc Evoy M., Anothaisintawee T., Sobhonslidsuk A., Attia J., Thakkinstian A. The association between cytokine gene polymorphisms and graft rejection in liver transplantation: a systematic review and meta-analysis // Transplant immunology. 2013. Vol. 28(1). Р. 62-70.
20. Rolly S. Chawla Y.K., Verma I., Kaur J. Association of interleukin-10 with hepatitis B virus (HBV) mediated disease progression in Indian population // The Indian journal of medical research. 2014. Vol. 139(5). P. 737 - 745.
21. Sepahi S. Pasdar A., Ahadi M., Gerayli S., Rostami S., Meshkat Z. Haplotype Analysis of Interleukin-10 Gene Promoter Polymorphisms in Chronic Hepatitis C Infection: A Case Control Study // Viral immunology. 2014. Vol. 27(8). Р. 398-403.
22. Sodsai P., Surakiatchanukul T., Kupatawintu P., Tangkitvanich P., Hirankarn N. Association of cytokine and cytokine receptor gene polymorphisms with the risk of chronic hepatitis B // Asian Pacific Journal of Allergy and Immunology. 2013. Vol. 31(4). Р. 277-285.
23. Wu J. F., Ni Y. H., Lin Y. T., Lee T. J., Hsu S. H., Chen H. L., Tsuei D. J., Hsu H. Y., Chang M. H. Human interleukin-10 genotypes are associated with different precore/core gene mutation patterns in children with chronic hepatitis B virus infection // The Journal of pediatrics. 2011. Vol. 158(5). Р. 808-813.
24. Yee L. J., Tang J., Herrera J., Kaslow R. A, van Leeuwen D. J. Tumor necrosis factor gene polymorphisms in patients with cirrhosis from chronic hepatitis C virus infection // Genes and immunity. 2000. Vol. 1(6). Р. 386-390.
25. Zhang T. C., Pan F. M., Zhang L. Z., Gao Y. F., Zhang Z. H., Gao J., Ge R., Mei Y., Shen B. B., Duan Z. H., Li X. A meta-analysis of the relation of polymorphism at sites− 1082 and− 592 of the IL-10 gene promoter with susceptibility and clearance to persistent hepatitis B virus infection in the Chinese population // Infection. 2011. Vol. 39(1). Р. 21-27.
26. Zhu Q. R., Ge Y. L., Gu S. Q., Yu H., Wang J. S., Gu X. H., Fei L. E., Dong Z. Q. Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection // Chinese medical journal. 2005. Vol. 118(19). Р. 1604-1609.
References:
1. Bedareva T.Yu. Izmeneniya tsitokinovogo statusa v ostrom periode kleshchevykh neyroinfektsiy u detey [Changes in status cytokines in the acute period of tick neuroinfections at children]. Byulleten' sibirskoy meditsiny [Bulletin of the Siberian medicine]. 2009. No 1. PP.10-14. [in Russian]
2. Bekenova N.B., Grjibovski A.M., Mukovozova L.A., Smail E. M., Tokaeva A. Z. Polimorfizm rs8193036 gena IL-17A v kazakhskoi populyatsii i ego svyaz' s produktsiei IL-17A u bol'nykh rozhei [rs8193036 polymorphism of IL-17A gene in a Kazakh population and its association with plasma IL-17A among erysipelas patients]. Ekologiya cheloveka [Human Ecology]. 2016. No. 4. PP.50-55. [in Russian]
3. Grjibovski A. M.. Ivanov S. V., Gorbatova M. A. Issledovaniya tipa «sluchay-kontrol'» v zdravookhranenii [Case-control studies in health sciences]. Nauka i Zdravookhranenie [Science & Healthcare]. 2015. No 4. PP. 5-17. [in Russian]
4. Emel'yanova A.N., Vitkovskii Yu.A., Kizhlo L.B., Kalinina E.N. Prognosticheskoe znachenie geneticheskogo polimorfizma molekul Il-2 (T330G), Il-10 (S819T), Il-10 (G1082A) u bol'nykh rozhei v Zabaikal'skom krae [Prognostic significance of genetic polymorphism of IL-2 (T330G), IL-10 (C819T), IL-10 (G1082A) in patients with erysipelas in Transbaikal regio] Dal'nevostochnyi zhurnal infektsionnoi patologii. 2012. No 21. PP. 159-163. [in Russian]
5. Ivanov V. V., Shipilov M. V. Interleykin-10 i ostrye respiratornye virusnye infektsii [Interleukin-10 and acute respiratory viral infections] Zdorov'e - osnova chelovecheskogo potentsiala-problemy i puti ikh resheniya [Health - the basics of the human potential - problems and solutions] 2011. No. 1. PP. 101-106. [in Russian]
6. Kostina E. M., Molotilov B.A., Baranova N. I., Levashova O. A. Osobennosti polimorfizma genov tsitokinov IL-4, IL-10, IL-17 A i FNO – α u bol'nykh s razlichnymi kliniko-patogeneticheskimi variantami infektsionno-zavisimoi bronkhial'noi astmy [Features of genes polymorphism of cytokine IL-4, IL-10, IL-17 A and TNF - α in patients with different clinical and pathogenic variants of an infectious-dependent asthma]. Allergologiya i immunologiya [Allergology and immunology] 2013. №1 (14). PP. 5-9. [in Russian]
7. Rizvanova F. F. i dr. Geneticheskaya diagnostika: polimorfizm genov tsitokinov [Genetic diagnosis: polymorphism of cytokine genes]. Prakticheskaya meditsina [Practical medicine] 2010. No. 6. PP.41-43.
8. Yurko E. V. Kharakteristika tsitokinovogo obmena u patsientov c ko-infektsiey VICh/KhGS [Characteristic of cytokine metabolism in patients with Hiv/HCV Co-infection]. Mezhnarodniy medichniy zhurnal [International Medical Journal]. 2014. No. 20. pp. 72-74. [in Russian]
9. Alvarado-Arnez L. E., Amaral E. P., Sales-Marques C., Durães S. M., Cardoso C. C., Nunes Sarno E., Pacheco A. G., Lana F. C., Moraes M. O. Association of IL10 Polymorphisms and Leprosy: A Meta-Analysis. PloS one. 2015, 9. pp.1-13.
10. Brooks D. G, Trifilo M., Edelmann K., Teyton L, Mc. Gavern D.B., Oldstone M.B. Interleukin-10 determines viral clearance or persistence in vivo. Nature medicine. 2006, 12(11). pp. 1301-1309.
11. da Silva N. M., Germano F.N., Vidalez –Braz B.M., Carmo Zanella Rd., dos Santos D.M., Lobato R., de Martinez A.M. Polymorphisms of IL-10 gene in patients infected with HCV under antiviral treatment in southern Brazil. Cytokine. 2015, 73(2). pp. 253-257.
12. Gao Q. J., Liu D.W., Zhang S.Y., Jia M., Wang L.M., Wu L.Y., Wang S.Y., Tong L.X. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol. 2009, 15(44). pp. 5610-5619.
13. Mc Guire W., Hill A.V., Allsopp C.E., Greenwood B.M., Kwiatkowski D. Variation in the TNF-α promoter region associated with susceptibility to cerebral malaria. Nature. 1994, 371(6497). pp. 508-511.
14. Meenakshi P., Ramya S., Shruthi T., Lavanya J., Mohammed H.H., Vijayalakshmi V, Sumanlatha G. Association of IL‐1β+ 3954 C/T and IL‐10‐1082 G/A Cytokine Gene Polymorphisms with Susceptibility to Tuberculosis. Scandinavian journal of immunology. 2013, 78(1). pp. 92-97.
15. Mira J. P., Cariou A., Grall F., Delclaux C., Losser M.R., Heshmati F., Cheval C., Monchi M., Teboul J.L., Riché F., Leleu G., Arbibe L., Mignon A., Delpech M., Dhainaut J.F. Association of TNF2, a TNF-α promoter polymorphism, with septic shock susceptibility and mortality: a multicenter study. Jama. 1999, 282(6). pp. 561-568.
16. Mosaad Y. M., Soliman O. E., Tawhid Z. E., Sherif D. M. Interferon‐gamma+ 874 T/A and Interleukin‐10‐1082 A/G Single nucleotide Polymorphism in Egyptian Children with Tuberculosis. Scandinavian journal of immunology. 2010, 72(4). pp. 358-364.
17. Nadel S., Newport M. J., Booy R., Levin M. Variation in the tumor necrosis factor-α gene promoter region may be associated with death from meningococcal disease. Journal of Infectious Diseases. 1996, 174(4). pp. 878-880.
18. Pereira V. A., Sánchez-Arcila J.C., Teva A., Perce-da-Silva D.S., Vasconcelos M. P., Lima C. A., Aprígio C. J., Rodrigues-da-Silva R. N., Santos D.O., Banic D. M., Bonecini-Almeida M. G., Lima-Júnior J. C., Oliveira-Ferreira J. IL10A genotypic association with decreased IL-10 circulating levels in malaria infected individuals from endemic area of the Brazilian Amazon. Malaria journal. 2015, 28(14). pp. 1-12.
19. Rattanasiri S., Mc Daniel D.O., Mc Evoy M., Anothaisintawee T., Sobhonslidsuk A., Attia J., Thakkinstian A. The association between cytokine gene polymorphisms and graft rejection in liver transplantation: a systematic review and meta-analysis. Transplant immunology. 2013, 28(1). pp. 62-70.
20. Rolly S. Chawla Y.K., Verma I., Kaur J. Association of interleukin-10 with hepatitis B virus (HBV) mediated disease progression in Indian population. The Indian journal of medical research. 2014, 139(5). pp. 737 - 745.
21. Sepahi S. Pasdar A., Ahadi M., Gerayli S., Rostami S., Meshkat Z. Haplotype Analysis of Interleukin-10 Gene Promoter Polymorphisms in Chronic Hepatitis C Infection: A Case Control Study. Viral immunology. 2014, 27(8). pp. 398-403.
22. Sodsai P. Surakiatchanukul T., Kupatawintu P., Tangkitvanich P., Hirankarn N. Association of cytokine and cytokine receptor gene polymorphisms with the risk of chronic hepatitis B. Asian Pacific Journal of Allergy and Immunology. 2013, 31(4). pp. 277-285.
23. Wu J. F., Ni Y. H., Lin Y. T., Lee T. J., Hsu S. H., Chen H. L., Tsuei D. J., Hsu H. Y., Chang M. H. Human interleukin-10 genotypes are associated with different precore/core gene mutation patterns in children with chronic hepatitis B virus infection. The Journal of pediatrics. 2011, 158(5). pp. 808-813.
24. Yee L. J., Tang J., Herrera J., Kaslow R. A., van Leeuwen D. J. Tumor necrosis factor gene polymorphisms in patients with cirrhosis from chronic hepatitis C virus infection. Genes and immunity. 2000, 1(6). pp. 386-390.
25. Zhang T. C., Pan F. M., Zhang L. Z., Gao Y. F., Zhang Z. H., Gao J., Ge R., Mei Y., Shen B. B., Duan Z. H., Li X. A meta-analysis of the relation of polymorphism at sites− 1082 and− 592 of the IL-10 gene promoter with susceptibility and clearance to persistent hepatitis B virus infection in the Chinese population. Infection. 2011, 39(1). pp. 21-27.
26. Zhu Q. R., Ge Y. L., Gu S. Q., Yu H., Wang J. S., Gu X. H., Fei L. E., Dong Z. Q. Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection. Chinese medical journal. 2005, 118(19). pp. 1604-1609..
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Бекенова Н.Б., Гржибовский А.М., Муковозова Л.А., Аукенов Н.Е. Полиморфизм гена ИЛ10 в положении -1082G/A у больных рожей / / Наука и Здравоохранение. 2016. №3. С. 34-45. Bekenova N.B., Grjibovski A.M., Mukovozova L.A., Aukenov N.Ye. Polymorphism of IL10 gene at -1082G/A position in patients with erysipelas. Nauka i Zdravookhranenie [Science & Healthcare]. 2016, 3, pp. 34-45. Бекенова Н.Б., Гржибовский А.М., Муковозова Л.А., Аукенов Н.Е. Тілмемен ауыратын науқастарда ИЛ10 генінің полиморфизмі (-1082G/A) / / Ғылым және Денсаулық сақтау. 2016. №3. Б. 34-45.Related publications:
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