INTERLEUKINS 10 AND 17A: THE RELATIONSHIP OF GENE POLYMORPHISMS TO DISEASE AND CYTOKINE LEVELS IN PATIENTS WITH BRUCELLOSIS IN THE KAZAKH POPULATION

Abstract

Актуальность: In predicting the clinical course of brucellosis, the participation of a genetic factor, in particular, gene
polymorphism of some cytokines, which are essential in the development of the disease, is not excluded. In this regard, the
search for genetic markers of susceptibility and features of the course of the disease among the alleles of cytokine gene
polymorphisms is of theoretical and practical interest.
Aim of research was to study the associations of the polymorphisms of the IL10 and IL17 genes with brucellosis, as well
as their connection with the production of the same cytokines in patients with brucellosis of Kazakh nationality.
Materials and Methods. In the case-control study, there were 89 patients and 422 healthy individuals of the Kazakh
population. Genotyping was performed by real-time PCR. The determination of cytokine levels in the blood plasma was
carried out by enzyme immunoassay.
Results. It has been established that susceptibility factors for brucellosis can be C allele and CC genotype (OR=4.42
and 7.32; 95% CI: 3.0–6.51 and 4.39–12.20, respectively), and T allele, CT, and TT genotypes of the rs8193036
polymorphism are resistance factors. Relationship was found with susceptibility to brucellosis in carriers of the G allele and
GG genotype of the rs2275913 polymorphism (OR=2.26 and 2.25; 95% CI: 1.51-3.38 and 1.40-3.61), and with resistance - A
allele and genotypes AA and AG. The concentration of IL-17A in patients with brucellosis with CC, CT and GG genotypes
was higher compared with the control group (p=0.054, 0.002 and 0.012, respectively).
Conclusions. Thus, IL17A gene polymorphisms can be associated with brucellosis disease, with the level of the
cytokine itself in the Kazakh population.