STUDYING IN EXPERIMENT OF INTRAOCULARSTRUCTURES AFTER INTRAVITREAL INTRODUCTIONOF CHITOSAN FILM, SATURATED WITH 5-FU

Abstract

Abstract. The treatment of proliferarive vitreoretinopathy (PVR) is a severe and actual problem
in ophthalmosurgery. At the present stage of PVR treatment new tendencies of application of antiproliferative agents on various carriers implants used during the intravitreal interventions were
outlined. Preparations, which action is directed on an ingibition of fabric formation, are widely used.
In particular: cytostatics. The famous representative of this group of preparations is 5 -FU.
In recent years is growing the interest of experts in preparations on the basis of chitin and
chitozan that collapses completely and acquired by an organism, possesses anti-inflammatory
action, high biocompatibility, improves processes of cell and fabrics regeneration
The aim of our research was to study in experiment a condition of intraocular structures after
intravitreal introduction of сhitosan film, saturated with 5-FU in dosages of 0,05 and 0,1 ml.
Methods: Data from 12 healthy rabbits (N=24). Weight: (from 5.0 lb to 7.0 lb) adult rabbits. The
experimental animals eyes were divided into 3 groups. The 1st main group of research included 9
right eyes, into which the chitosan film saturated with 0,05 ml 5-FU was entered. In the 2nd main
group respectively - the left eyes – the chitosan film saturated with 0,1 ml 5-FU. The control group
was made by 6 eyes injured intravitreally by a knife 19G. The chitosan films saturated with 5 -FU
were created in advance: the size - width of 1 mm, length of 8 mm, thickness of 0,5 mm is set;
sterilization of the films in the autoclave at a temperature of 180°C. The comparative description of
a histological picture of eye structures of the 1 and 2 main and control groups was carried out after
an enucleation of eyeballs on 7th, 14th and 28th days.
Results: When studying influence of chitosan film, saturated with 5-FU, on eye structures, in
terms from 7 to 28 days the macroscopic changes in both main groups wasn't revealed. Distinctions
were revealed at histologic research of enucleated eyes. In both groups the lack of inflammatory
reaction is noted, there was no formation of a connective tissue and macrophages. Toxicity neither
in the first, nor in the second main groups morphologically wasn't confirmed (there is no lysis of a n
internal boundary membrane). In the second main group the lysis of erythrocytes, which left the
vascular course during operation, was quicker and without development of connective tissue and
inflammation. In the first main group a small proliferative activity was noted, in the second was
absent.
Conclusion: Thus, according to results of morphological research, toxic action from intravitreal
injection of chitosan film, saturated with 5-FU (0,05 and 0,1 ml) in experiment wasn't observed.
Some proliferative activity was revealed after implantation of the chitosan film saturated with 0,05
ml 5-FU.