COMPARATIVE EVALUATION OF TYPING METHODSFOR E.COLI - MLST AND SEROTYPING

Abstract

Background and Objectives: Subspecies typing methods allow to carry out of genetic relationship
(clonality) analysis between strains within species and to establish a possible epidemiological link
between the source of infection, the factors of transmission. Identify and compare the results of
serological and multilocus sequencing typing data based on E.coli genomes.
Material and methods: Determination of serotypes and MLST types of E.coli have been done
based on genome-wide data using a web resource Center of Genomic Epidemiology
(www.genomicepidemiology.org). Discriminatory power and concordance of the results was calculated
using Rand and adj.Wallace indeces.
Results and Discussion: Research based on the genome-wide data E.coli with determination
MLST and serotypes demonstrated the same level of discriminating ability (95-96%). Concordance
between the results obtained by the two methods was 83.9% (95% CI 80,2-86,5). The predictive ability
for the MLST-based O:H serotypes was 76%, for serotypes using MLST data was 92.2% 

Conclusion: While resolution power of serotyping and MLST analysis were at same level (95-96%),
the concordance of the results was was 84%. There was very good conformity between some MLST
types and serotypes, at the same time, other MLST types were characterized with different serotypes.