MORPHOLOGY OF THE IN CHRONIC INTOXICATIONOF THE ORGANISM WITH SODIUM TETRABORATE

Abstract

Background: Aktobe region of the Republic of Kazakhstan is a biogeochemical region of chrome
and boron. There exist enough researches on the impact of various doses of chrome compounds on the
human body and animals, but researches on the impact of boron compounds in the organs and systems
of the body are almost absent.
Objective: to study the effects of sodium tetraborate on the morphology of the liver.
Methods: The study was conducted on 50 outbred white rats - males (10 intact, 20 control, 20
experimental). Experimental animals within 2 months daily intraperitoneally were administered sodium
tetraborate 150 mg / GFP saline in a volume of 1-2 ml. Control animals at the same time were injected
intraperitoneally with saline solution in a volume of 1-2 ml. The materials of the study were pieces of
liver. After the slices of liver tissue processors 7-8 microns thick were stained with hematoxylin - eosin
and Gieson baths. Morphometry was performed by network 40X40 square microns (250 points).  

Results: In 2 months of chronic intoxication with sodium tetraborate blood stasis – filling of central
vein with blood, dilation of sinusoidal capillaries, degeneration mainlyni light hepatocytes located und
central vein – was being developed; the number of fibroblasts and collagen fibers around liver triade
increased; amitosis of hepatocytes nuclei was noted; the increase of the number of binuclear cells (in
control 6,2±0,41%, in experiment 12,7±0,75%), polyploidy cells (in control 1,2±0,02%, in experiment
4,3±0,27%). In this case there was decrease of weight index (in control 41,7±2,14%, in experiment
36,3±2,53%).
Conclusion: Chronic intoxication of a rat with sodium tetraborate results into: degeneration of
hepatocytes, blood stasis in the hepatic venous vascular segments, an increase in the number of dualcore and polyploid hepatocytes.