IMMUNE STATUS, ANTIOXIDANT PROTECTION SYSTEM AND ENZYMES OF METABOLISM OF PURINE NUCLEOTIDES IN SYMPATHETIC HYPERACTIVATION
Introduction. It is known that activation of the sympathoadrenal system aggravates the course of coronary heart disease. And elevated levels of catecholamines are a risk factor for the development of repeated myocardial infarction and sudden death.
Purpose of the study. With hyperadrenalinemia caused by the administration of adrenaline to animals at a dose of 4 mg / kg 60 minutes before the study, to study the status of the immune status, the parameters of the antioxidant defense system and the enzymes of the purine nucleotide metabolism.
Material and methods. Study design: experimental. In the blood, in the heart and liver of mongrel white rats aged 3-3.5 months weighing 160-180, the status of the immune status, the activity of glutathione peroxidase (GPO), glutathione reductase (GR), catalase, adenosine deaminase (AD), AMP deaminase (AMPD), 5'-nucleotidase (5'H) and the level of malonic dialdehyde (MDA) and diene conjugates (DC).
The results of the study were processed using the t-test of the Student. The paper presents the arithmetic mean ± mean error (X ± m). Differences were considered significant at p <0,05.
Results. It was found that with hyperadrenalinemia, the functional interrelation between the T and B immunity links is enhanced, the total number of leukocytes and lymphocytes increases, the number of T suppressors is increased, the activity of AMPD, AD, 5'H and GPO increases, the level of DC increases, the inhibition of migration of leukocytes decreases (RTML) and the NCT test. In the heart, hyperadrenalinemia is accompanied by activation of AD, AMPD, catalase, an increase in the level of MDA, a decrease in the activity of 5'H, and an increase in the ratio of the activities of AD + AMPD / 5'H enzymes towards the enhancement of deamination of adenosine and AMP. In the liver, the injection of epinephrine causes an increase in the level of MDA and DC, the activation of catalase and enzymes of the metabolism of purines AD, AMPD and 5'H.
Conclusions. With sympathetic hyperactivation, the functional interrelation of the T- and B-links of immunity is enhanced, shifts approximate to the state of oxidative stress occur, which is manifested by activation of GPO, catalase and enzymes of the metabolism of purine nucleotides AD, AMPD and 5'H, an increase in the level of MDA and DC.
Salavat O. Tapbergenov, http://orcid.org/0000-0003-0703-7458
Bаkhytbек S. Sovetov, http://orcid.org/0000-0001-9291-558Semey State Medical University, Semey, Kazakhstan.
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