METABOLIC EFFECTS OF COMBINED INTEGRATION OF ADENOSINE AND ADENOSINE MONOPHOSPHAT IN HYPERADENALEMEMIA
Introduction. It is known that adenosine as a metabolite of adenosune monophosphate (AMP) has a variety of functional and metabolic effects aimed at maintaining homeostasis. Adenosine plays a particularly important role in the adaptation of the myocardium to ischemia and hypoxia. By exerting an antiarrhythmic effect on the heart, adenosine slows the atrioventricular conduction induced by catecholamines.
Purpose of the study. To study the effects of the combined effect of the adenosine + AMP complex on the immune status, on the activity of antioxidant protection enzymes and purine nucleotide metabolism, on the level of MDA and DC in the blood, in the heart and liver in hyperadrenalinemia.
Material and methods. Study design: experimental. In the blood, in the heart and liver of white rats against the background of hyperadrenalinemia, created in / b adrenaline injection at a dose of 0.4 mg per 100 g of body weight 60 minutes before the study, the effect of the adenosine + AMP complex at a total dose of 1000 μg on the immune status, On the activity of glutathione peroxidase (GРO), glutathione reductase (GR), catalase, adenosine deaminase (AD), AMP-deaminase (AMPD), 5'-nucleotidase (5'H), on the level of malonic dialdehyde (MDA) and diene conjugates (DC).
The results of the study were processed using the t-test of the Student. The paper presents the arithmetic mean ± mean error (X ± m). Differences were considered significant at p <0.05.
Results. In hyperadrenalemia, the introduction of the adenosine and AMP complex reduces the total number of leukocytes, T-lymphocytes and T-suppressors, increases T-lymphocytes and RTML, increases the activity of the enzymes of metabolism of purines AD, AMPD, 5'H and GR, reduces the activity of GPO, catalase and the level MDA in the blood
In the heart with sympathetic hyperactivation, the complex of adenosine and AMP leads to the activation of AD and AMPD, to an increase in the ratio of the activities of AD + AMPD / 5'H towards the catabolism of adenosine and AMP, causes a decrease in the amount of MDA and DC, and the activity of GR, GPO and catalase, which indicates a decrease in peroxidation processes in this organ.
In the liver with hyperadrenalinemia, the adenosine and AMP complex reduces the level of MDA and DC, and the activity of GAP and catalase decreases accordingly, thereby reducing the level of oxidative stress caused by adrenaline.
Conclusions. To correct the changes in the antioxidant defense system, the activity of the purine metabolism enzymes and immune responses observed in hyperadrenalemia and oxidative stress of any origin, a complex of adenosine and AMP can be used.
Salavat O. Tapbergenov 1, http://orcid.org/0000-0003-0703-7458
Bаkhytbек S. Sovetov 1, http://orcid.org/0000-0001-9291-558
Аrtur Т. Tapbergenov 2, http:// orcid.org/0000-0002-8533-1997
Elina Hahn 3, http://orcid.org/0000-0002-1530-853X
1 Semey State Medical University, Semey, Kazakhstan.
Department of Biochemistry and Chemical disciplines;
2 Cardiosurgery Center, HELIOS Clinic, Siegburg, North Rhine-Westphalia, Germany;
3 Julius-Maximilian-University of Wurzburg, Medical Faculty, Wurzburg, Bavaria, Germany.
1. Vlasova S.N., Shabunina E.I., Pereslegina I.A. Aktivnost' glutationzavisimykh fermentov eritrotsitov pri khronicheskikh zabolevaniyakh pecheni u detei. [Activity of glutathione-dependent erythrocyte enzymes in chronic liver diseases in children]. Laboratornoye delo [Laboratory work]. 1990, №8, pp. 19-22. [in Russian]
2. Gavrilov V.B., Gavrilov A.R., Hmara A.F. Izmerenie dienovykh kon'yugatov v plazme po ul'trafioletovomu pogloshheniyu geptanovykh i izopropil'nykh ekstraktov [Measurement of diene conjugates in plasma by ultraviolet absorption of heptane and isopropyl extracts]. Laboratornoye delo [Laboratory work]. 1988, №2, рр. 60-64. [in Russian]
3. Korolyuk M.A., Ivanova L.I., Majorova I.T. Metod opredeleniya aktivnosti katalazy. [Method for the determination of catalase activity]. Laboratornoe delo [Laboratory work]. 1988, № 1, рр. 16-18. [in Russian]
4. Kozlovskii V.I., Zinchuk V.V, Stankevich P.B, Hlopickij S. Rol' adenozina v regulyatsii funktsii serdechno-sosudistoi sistemy [The role of adenosine in the regulation of cardiovascular functions]. Zhurnal Grodnenskogo gosudarstvennogo medicinskogo universiteta [Journal of the Grodno State Medical University] 2007, Vyp.1. №17, P.49-53 [in Russian]
5. Makolkin V.I., Ahmedova O.O., Buval'tsev V.I. i dr. Klinicheskie i metabolicheskie effekty kardioselektivnykh β-blokatorov nebivolola i metoprolola u bol'nykh arterial'noi gipertoniei i ishemicheskoi bolezn'yu serdtsa v sochetanii s sakharnym diabetom 2-go tipa [Clinical and metabolic effects of cardioselective β-block of nebivolol and metoprolol tori in patients with arterial hypertension and ischemic heart disease in combination with type 2 diabetes mellitus]. Kardiologiya [Cardiology]. 2003. №2, P.40-43. [in Russian]
6. Nagoev B.S., Shubich M.G. Znachenie testa vosstanovleniya nitrosinego tetrazoliya dlya izucheniya funktsional'noi aktivnosti leikotsitov. [The value of the test for the reduction of nitrosine tetrazolium for the study of the functional activity of leukocytes]. Laborotornoye delo [Laboratory work]. 1981, №4, рр.195-198. [in Russian]
7. Tapbergenov S.O., Tapbergenova S.M. Diagnosticheskoe znachenie opredeleniya aktivnosti adenilatdezaminazy syvorotki krovi. [Diagnostic value of determination of serum adenylate deaminase activity]. Laboratornoe delo [Laboratory work]. 1984, 2, рр.104-107. [in Russian]
8. Tapbergenov S.O., Tapbergenov T.S. Fermenty metabolizma purinovykh nukleotidov v otsenke funktsional'noi polnotsennosti immuniteta [The enzymes of the metabolism of purine nucleotides in the evaluation of the functional usefulness of immunity]. Biomeditsinskaya khimiya [Biomedical chemistry]. 2005, Vyp. 51. №2, рр.199-205 [in Russian]
9. Tapbergenov S.O., Tapbergenov T.S. Fermenty metabolizma purinovykh nukleotidov i immunnyi status pri stressornykh sostoyaniyakh raznogo proishozhdeniya [The enzymes of the metabolism of purine nucleotides and the immune status under stress conditions of different origin]. Uspekhi sovremennogo estestvoznaniya. [Advances in modern natural science]. 2009, №7. pp.92-93. [in Russian]
10. Tapbergenov S.O., Tapbergenov A.T. Vliyanie simpaticheskoi giperaktivatsii i adrenoblokatora metoprolola na immunnyi status i aktivnost' fermentov purinovykh nukleotidov [Effect of sympathetic hyperactivation and adrenoblocker metoprolol on immune status and activity of purine nucleotide enzymes. Mezhdunarodnyi zhurnal eksperimental'nogo obrazovaniya [International Journal of Experimental Education]. 2013, № 3, рр. 147-150. [in Russian]
11. Tapbergenov S.O., Sovetov B.S., Tapbergenov A.T. Osobennosti vozdeistviya adenozina, AMF i giperadrenalinemii na immunnyi status, fermenty metabolizma purinovykh nukleotidov i sistemu antioksidantnoi sistemy. [Peculiarities of the effect of adenosine, AMP and hyperadrenalinemia on the immune status, enzymes of the purine nucleotide metabolism and the antioxidant system]. Biomeditsinskaya khimiya [Biomedical chemistry]. 2016, V.62, № 6. рр. 645-649 [in Russian]
12. Tereschenko S.N., Kositsyna I.V., Dzhaiani N.A. Vse li my znaem ob osobennostyakh metoprolola v lechenii ishemicheskoi bolezni serdtsa? [Do we all know the peculiarities of metoprolol in the treatment of coronary heart disease?]. Kardiologiya. [Cardiology], 2005, № 4. P. 98-101. [in Russian]
13. Belardinelli L., Giles W.R. Ionic mechanisms of adenosine actions in pacemaker cells from rabbit heart. J. Physiol., 1988. Vol. 405, P. 615-633.
14. Clausen J.E. Tuberculin-induced migration inhibition of human peripheral leucocytes in agarose medium. Acta Allergol., 1971, Vol. 26(1), рр. 56-80.
15. Ehlenberger A.G., Mc.Williams M., Phillips-Quagliata J.M. Immunoglobulin-bearing and complement-receptor lymphocytes constitute the same population in human peripheral blood. J Clin Invest. 1976, Vol. 57 (1), рр. 53–56. 80.
16. Lerman B.B., Belardinelli L. Cardiac electrophysiology of adenosine. Basic and clinical concepts. Circulation.1991. Vol. 83, P.1499-1509.
20. Limatibul S., Shore A., Dosch H.M., Gelfand W. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. J. Clin. and Exp. Immunol., 1978, Vol 33, (3), Р.503-510.
17. Limatibul S., Shore A., Dosch H.M., Gelfand W. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. J. Clin. and Exp. Immunol.,1978, Vol 33, (3), рр. 503-510.
18. Naamani O., Chaimovitz C., Douvdevani A. Pharmacological preconditioning with adenosine A(1) receptor agonist suppresses cellular immune response by an A(2A) receptor dependent mechanism. Int Immunopharmacol. 2014, Vol. 20, №1, рр. 205-212
19. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J. Exp., Med., 1972, Vol. 136, рр. 207-209.
20. Pelleg A., Mitsuoka T., Mazgalev T.,Michelson E.L. Vagal component in the chronotropic and dromotropic actions of adenosine and ATP. Prog Clin Biol Res. 1987, Vol. 230, рр. 375-384.
21. Uchiyama M., Mihara M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Analyt. Biochemia. 1978, №86, рр. 271-278.