ON THE PROBLEM OF THE AGE OF RATS USED FOR ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY STUDIES

Introduction: After irradiation for the rehabilitation of persons affected by uncontrolled radiation exposure (from several days to several tens of years) for the purpose of reconstruct the absorbed doses after a long time applies a method that based on electron paramagnetic resonance spectroscopy (EPR – dosimetry). This method is a very effective tool for retrospective individual dosimetry, because it makes possible to measure materials that are part of the human body [5, 6, 13, 14, 18, 20]. ESR measures the quantity of stable radicals, created due to radiation exposure to tooth enamel.

Aim of research: Determination of the feasibility of conducting studies on the measurement of the absorbed doses use rat’s enamel.

Materials and methods. The experimental study was carried out on 38 white laboratory rats of the «Wistar» breed. The mammals were separated on 4 groups: I - 56MnO2, II - MnO2, III - 60Co and IV – control, its weight were 220 – 330 gr.

The first two groups were beta-irradiated in the IVG.1M («Baikal») nuclear reactor, which is located 70 km from the city of Kurchatov. The group of third was gamma-irradiated in the Semey Oncology Center. After the animals’ decapitation the biological materials (teeth) were delivered to the laboratory of the Department of Applied Physics of the Okayama University of Science (Japan).

Standard parameters of EPR spectra measurements were used for the equipment (JEOL JES-FA100).

Results: During the measurements it was shown that the samples of rats dental enamel showed no presence of the EPR signal, after which it was determined that the age of enamel formation is insufficient for such studies and requires more accurate and qualitative approach to the selection of the rats.

Aisulu Zh. Saimova 2, http//orcid.org/0000-0002-9564-732X

Sholpan Sh. Abralina 2, httр://оrcid.оrg/0000-0002-3681-3332

Nurlan B. Sayakenov 2, httр://оrcid.оrg/0000–0002–5082–7554

Valеriy F. Stерanеnkо 3, http://orcid.org/0000-0002-3541-0515

Shin Toyoda 4, http://orcid.org/0000-0002-1524-7167

Masaharu Hоshi 5, httр://оrcid.оrg/0000–0001–6978–0883

Nariaki Fujimoto 5, httр://оrcid.оrg/0000–0002–8570–4001

Nailya Zh. Chaizhunusоva 2, httр://оrcid.оrg/0000–0002–6660–7118

Tоlеbay K. Rаkhyрbеkоv 2, httр://оrcid.оrg/0000–0002–5699–3086

1 International Department of Nuclear Physics, New Materials and Technology, L.N. Gumilyov Eurasian National University, Astana, Kazakhstan;

2 Semey State Medical University, Semey, Kazakhstan;

3 A. Tsyb Medical Research Radiological Center (MRRC) – National Medical Research Radiological Center Ministry of Health of Russian Federation, Obninsk, Russia;

4 Okayama University of Science, Okayama, Japan;

5 Hiroshima University, Hiroshima, Japan.

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Bibliography link

Zhumadilov К.Sh., Saimova A.Zh., Abralina Sh.Sh., Sayakenov N.B., Stерanеnkо V.F., Toyoda Sh., Hоshi M., Fujimoto N., Chaizhunusоva N.Zh., Rаkhyрbеkоv T.K. On the problem of the age of rats used for electron paramagnetic resonance spectroscopy studies. Nauka i Zdravookhranenie [Science & Healthcare]. 2017, 2, pp. 105-114.