PLASMA METABOLOME PROFILING OF THE KAZAKHS
Introduction: The determination of metabotype variations can be used to predict disease risk and diagnosis, understand molecular pathophysiology, interpret the understanding of environmental and lifestyle influences, develop and evaluate drug efficacy, toxicity, and adverse reactions.
Aim: In this study metabolic differences among adults living in Kazakhstan are assessed to identify and characterize the metabolic profiles and profiles of clinical biomarkers.
Materials and Methods: Observational trans-sectional study of healthy Kazakhs. To perform the tasks, metabolom study of plasma was conducted among 74 Kazakh nationality study participants. The study was carried out on a platform based on tandem technology of ultra-high liquid chromatography and mass spectroscopy (Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectroscopy (UPLC-MS / MS)). The determination of the level of biochemical indices in blood serum was performed on a biochemical analyzer Cobas 6000, Roche Diagnostics. The necessary logarithmic transformation and ANOVA variance analysis, a two-sample Welch t-test, were performed to determine the bio-compounds that differed significantly between the experimental groups.
Results: The study was conducted to gain an understanding of the metabolic changes that occur in young adults and adults over 45 years. As a result of 74 participants, 853 different biochemical indicators of the main pathways for the metabolism of amino acids, pethids, nucleotides, carbohydrates, cofactors and vitamins, xenobiotics, lipid and energy metabolism were identified.
Conclusion: The changes in several known metabolites and various prospective metabolic pathways in the group older than 45 years are found compared to a group of young people. Metabolic differences included changes in metabolites associated with the metabolism of fatty acids, steroidogenesis (steroid hormone biosynthesis), secondary carnitine metabolism, inflammation and oxidative stress.
Aliya Abilmazhinova 1,
Ulan Kozhamkulov 1, http://orcid.org/0000-0002-9782-7631
Joseph Lee 2,3, Scopus ID 56108217700
Ainur Akilzhanova 1, http://orcid.org/0000-0001-6161-8355
1 Laboratory of Genomic and Personalized Medicine, Center for Life Sciences,
National Laboratory Astana, Nazarbayev University,
Astana, Kazakhstan;
2 The Taub Institute for Research in Alzheimer’s Disease and the Aging Brain and the G.H. Sergievsky Center at Columbia University,
New York, NY, USA;
3 Department of Epidemiology, School of Public Health, Columbia University,
New York, NY, USA.
1. Abylaiuly Zh., Dzhusipov A.K., Berkinbayev S.F., Shalharova Zh.S., Oshakbayev K.P., Bekzhigitov S.B., Abseitova S.R., Moldabek G.K., Maimakov A. A., Akhmetzhanov N. A., Djunusbekova G.A., Musagaliyeva A.T., Akhmentayeva D.A. Proyekt konsensusa po diagnostike i vedeniyu metabolicheskogo sindroma v Respublike Kazakhstan. Razrabotchik po rezul'tatam rezolyutsii Respublikanskoy nauchno-prakticheskoy konferentsii «Metabolicheskiy sindrom. Realii i perspektivy v kardiologii i endokrinologii» [Draft consensus on the diagnosis and management of metabolic syndrome in the Republic of Kazakhstan. Developed by the results of the resolution of the Republican Scientific and Practical Conference "Metabolic Syndrome. Realities and perspectives in cardiology and endocrinology”]. Terapevticheskiy vestnik [Therapeutic Herald]. 2008. 4 (20). P. 27-32. [in Russian]
2. Benberin V.V., Karabayeva R.Zh. Metabolicheskiy sindrom: voprosy izucheniya i perspektivy razvitiya issledovaniy [Metabolic syndrome: study questions and research development prospects]. Vestnik Meditsinskogo tsentra Upravleniya delami Prezidenta Respubliki Kazakhstan [Bulletin of the Medical Center of the Department of Affairs of the President of the Republic of Kazakhstan]. 2012. 1. P.3-5. [in Russian]
3. Adamski J., Suhre K. Metabolomics platforms for genome wide association studies – linking the genome to the metabolome. Current opinion in biotechnology. 2013. 24. P.39-47.
4. Akanov A.A., Tulebaev K.A., Eshmanova A.K., Chaĭkovskaia V.V., Abikulova A.K., Kalmakhanov S.B., Mansharipova A.T. Analysis of condition and prospects in geriatric care of population of Kazakhstan. Adv Gerontol. 2014. 27(3). P.589-595.
5. Burke G.L., Bertoni A.G., Shea S., Tracy R., Watson K.E., Blumenthal R.S., Chung H., Carnethon M.R. The impact of obesity on cardiovascular disease risk factors and subclinical vascular disease: the Multi-Ethnic Study of Atherosclerosis. Archives of internal medicine. 2008. 168(9). P.928-935.
6. Chrostowska M., Szyndler A., Paczwa P., Narkiewicz K. Impact of abdominal obesity on the frequency of hypertension and cardiovascular disease in Poland - results from the IDEA study (international day for the evaluation of abdominal obesity). Blood pressure. 2011. 20(3). P.145-152.
7. Fox K.A., Despres J.P., Richard A.J., Brette S., Deanfield J.E. Does abdominal obesity have a similar impact on cardiovascular disease and diabetes? A study of 91,246 ambulant patients in 27 European countries. European heart journal. 2009. 30(24). P.3055-3063.
8. Jordan K.W., Nordenstam J., Lauwers G.Y., Rothenberger D.A., Alavi K., Garwood M., Cheng L.L. Metabolomic characterization of human rectal adenocarcinoma with intact tissue magnetic resonance spectroscopy. Diseases of the Colon & Rectum. 2009. 52 (3). P.520–525.
9. Lavie C.J., Milani R.V., Ventura H.O. Obesity and cardiovascular disease: risk factor, paradox, and impact of weight loss. Journal of the American College of Cardiology. 2009. 53(21), P.1925-1932.
10. Oshakbayev K.P., Kenneth Alibek, Ponomarev I.O., Dukenbayeva B.A., Uderbayev N.N., Oshakbayev P., Mustafin H. The heating value of a different location of human body lipids. Global Journal of Medical Research. 2014. 13(7). P.19-23.
11. Oshakbayev K.P., Kenneth Alibek, Ponomarev I.O., Uderbayev N.N., Dukenbayeva B.A., Gazaliyeva M., Oshakbayev P., Kaliyeva Sh. Body fats accumulate metabolic products: physical and chemical analysis in vitro. American Journal of Medical and Biological Research. 2014. 2(1). P.5-11.
12. WHO. Highlights on health in Kazakhstan. 2005. 38p.
13. Zalesin K.C., Franklin B.A., Miller W.M., Peterson E.D., McCullough P.A. Impact of obesity on cardiovascular disease. The Medical clinics of North America. 2011. 95(5). P. 919-937