Science & Healthcare

Introduction: The determination of metabotype variations can be used to predict disease risk and diagnosis, understand molecular pathophysiology, interpret the understanding of environmental and lifestyle influences, develop and evaluate drug efficacy, toxicity, and adverse reactions.

Aim: In this study metabolic differences among adults living in Kazakhstan are assessed to identify and characterize the metabolic profiles and profiles of clinical biomarkers.

Materials and Methods: Observational trans-sectional study of healthy Kazakhs. To perform the tasks, metabolom study of plasma was conducted among 74 Kazakh nationality study participants. The study was carried out on a platform based on tandem technology of ultra-high liquid chromatography and mass spectroscopy (Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectroscopy (UPLC-MS / MS)). The determination of the level of biochemical indices in blood serum was performed on a biochemical analyzer Cobas 6000, Roche Diagnostics. The necessary logarithmic transformation and ANOVA variance analysis, a two-sample Welch t-test, were performed to determine the bio-compounds that differed significantly between the experimental groups.

Results: The study was conducted to gain an understanding of the metabolic changes that occur in young adults and adults over 45 years. As a result of 74 participants, 853 different biochemical indicators of the main pathways for the metabolism of amino acids, pethids, nucleotides, carbohydrates, cofactors and vitamins, xenobiotics, lipid and energy metabolism were identified.

Conclusion: The changes in several known metabolites and various prospective metabolic pathways in the group older than 45 years are found compared to a group of young people. Metabolic differences included changes in metabolites associated with the metabolism of fatty acids, steroidogenesis (steroid hormone biosynthesis), secondary carnitine metabolism, inflammation and oxidative stress.