Online ISSN: 3007-0244,
Print ISSN:  2410-4280
APPLICATION OF MALDI-MS MASS SPECTROMETRY FOR THE DETECTION OF SARS-COV-2 IN NASOPHARYNGEAL SWABS. SYSTEMATIC REVIEW
Relevance. The global spread of COVID-19, which has claimed millions of lives, has shown serious problems in various areas of healthcare in most countries, including Kazakhstan. One of the main tasks in the fight against the pandemic was to choose an appropriate method for diagnosing COVID-19. Reverse transcription-PCR (RT-PCR) has been recognized as the gold standard for virus detection. However, studies have demonstrated the effectiveness of matrix laser desorption/ionization mass spectrometry (MALDI-MS) and machine learning analysis to detect SARS-CoV-2 in nasal swabs. Aim. Analysis of the literature on the use of MALDI-MS mass spectrometry for the detection of SARS-CoV-2 in human nasal swabs. Search strategy. An analysis was made of full-text publications in English for the period 2019-2021, in the Medline, Scopus databases. The following keywords and their combinations were requested: SARS-CoV-2 and MALDI and “mass spectrometry” and ms. Forty-seven articles were identified, of which 37 were included in the study for review. Inclusion criteria: Articles in English for the last 3 years (2019-2021) describing the experience of using MALDI-TOF MS mass spectrometry for the detection of SARS-CoV-2 in nasal swabs were analyzed and processed. Exclusion Criteria: Articles that do not describe the MALDI-MS method or a biomaterial other than a nasal swab. Results and conclusions. Based on the results of the studied literature, the use of mass spectrometry is possible as an alternative method of RT-PCR.
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Category of articles: COVID-19 - Topical Subject

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Kadyrova I.A., Sultanbekova A.A., Barkhanskaya V.I., Kolesnichenko S.I., Kolesnikova E.А., Lavrynenko А.V., Korshukov I.V., Bekov Ye.K., Yegorov S.V., Babenko D.B. Application of MALDI-MS mass spectrometry for the detection of SARS-CoV-2 in nasopharyngeal swabs. Systematic review // Nauka i Zdravookhranenie [Science & Healthcare]. 2022, (Vol.24) 5, pp. 36-44. doi 10.34689/SH.2022.24.5.005

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