CYTOKINE GENE POLYMORPHISM WITH MYOCARDIAL INFARCTION IN SUBJECTS OF RUSSIAN NATIONALITY
Objective: This article considers the study of the frequency of allelic variants and genotypes of cytokines (TNF-α, IL-10, IL-6) in patients of acute myocardial infarction with segment elevation ST (STEMI) in subjects of Russian nationality.
Materials and Methods: The polymorphism of cytokine genes was examined by restriction analysis of specific areas of the genome amplification products in 41 (69.5%) men and 18 (30.5%) women of Russian nationality. Control group was consisted of 75 healthy subjects (donors of Russian nationality). In all subjects were studied single nucleotide polymorphism SNP-gene promoter region TNF-α (G-308A), IL-10 (G-1082A), IL-6 (G-174C). STEMI diagnosis was for accordance with the classification of the European Society of Cardiology (ESC). All patients were divided into 2 groups, where the group of uncomplicated STEMI amounted to 34 (57.6%) patients who did not have complications, both in hospital period and posthospital period. The second group was consisted of 25 (42.4%) patients with complicated course of STEMI.
Results: In this paper shows, the association of the homozygous genotype minor type A / A in the position of G-308A promoter region of the TNFα gene with complicated STEMI (OR = 6,72, p<0.05). It was revealed that the minor type homozygous genotype C / C position of G-174C promoter region of the gene IL-6 is also associated with complicated STEMI (OR = 3,25, p<0.05). According to our results, there were no significant differences in the frequency of alleles and genotypes of polymorphism G-1082A gene IL-10, depending on the course of STEMI. Thus, polymorphisms of cytokine genes could be considered as a possible genetic risk factor for morbidity in patients with STEMI of Russian nationality (East Kazakhstan region of the Republic of Kazakhstan), after further study, with a significant increase the size of sample.
Alfiya О. Aimagambetova 1*, http://orcid.org/0000-0003-1496-3281
Lyudmila K.Karazhanova 1, http://orcid.org/0000-0002-4719-6034
Arkady-Avi Kotlyar 2-3, http://orcid.org/0000-0002-5070-0000
Meiramgul O. Tokbulatova 1, http://orcid.org/0000-0003-3859-4812
1 Semey State Medical University, Department of therapy internship,
Semey, Kazakhstan;
2 Hospital «Kaplan», Rehovot, Israel;
3 Hospital «Integrative medicine», Rishon Le Zion, Israel
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4. Casas J.P. et al. Investigating the genetic determinants of cardiovascular disease using candidate genes and meta-analysis of association risk factors. Intern J Molec Med. 2007. Vol. 19. pp. 129-41.
5. Heiskanen M. et al. Polymorphism in the IL10 promoter region and early markers of atherosclerosis: The cardiovascular risk in young finns study. Atherosclerosis. 2010. Vol. 208. P. 190–196.
6. Hoffstedt J. et al. Excessive fat accumulation is associated with the TNFa -308G/A promoter polymorphism in women but not in men. Diabetologia. 2000. Vol. 43. pp. 117-120.
7. Humphries S.E., Luong L.A., Ogg M.S., et al. The interleukin-6 -174 G/C promoter polymorphism is associated with risk of coronary heart disease and systolic blood pressure in healthy men. European Heart Journal. 2001. Vol. 22. pp. 2243–2252.
8. Lev E.I., Komowski R., Vaknin-Assa H. et al. Comparison of the predictive value of four different risk scores for outcomes of patients with ST-elevation acute myocardial infarction undergoing primary percutaneous coronary intervention. Am. J. Cardiol. 2008. Vol. 102. pp.6-11.
9. Meldrum D. R. Tumor necrosis factor in the heart. Amer. J. Physiol. Regul. Integr. Comp. Physoil. 1998. Vol. 274. pp. 557-595.
10. Skoog T., Dicht W., Boquist S. et al. Plasma tumour necrosis factor α and early carotid atherosclerosis in healthy middle-aged men. Eur. Heart J. 2002. Vol. 23. pp. 376-383.
11. Thygesen K., Alpert J.S., White H.D. et al. Universal definition of myocardial infarction. Rational Pharmacotherapy in Cardiology. 2008. №5. pp. 91-105.
12. Thygesen K., Alpert J.S., Jaffe A.S. et al. Third universal definition of myocardial infarction. Circulation. 2012. Vol. 126, №16. pp. 2020-2035.
13. Young J., Libby P., Schonbeck U. Cytokines in the pathogenesis of atherosclerosis. Thrombosis and Haemostasis. 2002. Vol. 88(4). pp. 554–567.
14. The official WHO site. Electronic resource. Access http://www.who.int/chp/chronic_disease_report/ (10.01.2015).
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Аймагамбетова А.О., Каражанова Л.К., Котляр А., Токбулатова М.О. Полиморфизм генов цитокинов при инфаркте миокарда у лиц русской национальности / / Наука и Здравоохранение. 2016. № 5. С. 121-130. Aimagambetova A.О., Karazhanova L.K., Kotlyar A., Tokbulatova M.O. Cytokine gene polymorphism with myocardial infarction in subjects of russian nationality. Nauka i Zdravookhranenie [Science & Healthcare]. 2016, 5, pp. 121-130. Аймагамбетова А.О., Каражанова Л.К., Котляр А., Токбулатова М.О. Ұлты орыс науқастарының миокард инфаркты кезіндегі цитокиндер генінің полиморфизмы / / Ғылым және Денсаулық сақтау. 2016. № 5. Б. 121-130Related publications:
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