MORPHOLOGY OF THE IN CHRONIC INTOXICATION OF THE ORGANISM WITH SODIUM TETRABORATE
Background: Aktobe region of the Republic of Kazakhstan is a biogeochemical region of chrome and boron. There exist enough researches on the impact of various doses of chrome compounds on the human body and animals, but researches on the impact of boron compounds in the organs and systems of the body are almost absent.
Objective: to study the effects of sodium tetraborate on the morphology of the liver.
Methods: The study was conducted on 50 outbred white rats - males (10 intact, 20 control, 20 experimental). Experimental animals within 2 months daily intraperitoneally were administered sodium tetraborate 150 mg / GFP saline in a volume of 1-2 ml. Control animals at the same time were injected intraperitoneally with saline solution in a volume of 1-2 ml. The materials of the study were pieces of liver. After the slices of liver tissue processors 7-8 microns thick were stained with hematoxylin - eosin and Gieson baths. Morphometry was performed by network 40X40 square microns (250 points).
Results: In 2 months of chronic intoxication with sodium tetraborate blood stasis – filling of central vein with blood, dilation of sinusoidal capillaries, degeneration mainlyni light hepatocytes located und central vein – was being developed; the number of fibroblasts and collagen fibers around liver triade increased; amitosis of hepatocytes nuclei was noted; the increase of the number of binuclear cells (in control 6,2±0,41%, in experiment 12,7±0,75%), polyploidy cells (in control 1,2±0,02%, in experiment 4,3±0,27%). In this case there was decrease of weight index (in control 41,7±2,14%, in experiment 36,3±2,53%).
Conclusion: Chronic intoxication of a rat with sodium tetraborate results into: degeneration of hepatocytes, blood stasis in the hepatic venous vascular segments, an increase in the number of dual-core and polyploid hepatocytes.
A. K. Berdalinova, T. Zh. Umbetov, Zh. E. Komekbai
West Kazakhstan Marat Ospanov State medical university, Aktobe c., Kazakhstan
Литература:
1. Афанасьев Ю. И. Юрина Н. А. Печень // Гистология, цитология, эмбриология (учебник). Москва, 2012. С.595-606.
2. Бекетова Т. П., Секамова С. М. К вопросу структурной гетерогенности гепатоцитов // III Всесоюзная Конференция по патологии клетки. (Тез. докл). 1982. С.101-102.
3. Борисов А. И. Гигиенические аспекты охраны окружающей среды // Гигиена и санитария. 1976. №1. С.11-16.
4. Вербицкая Г. В. Актуальные вопросы гигиены населенных мест // Гигиена и санитария. М., 1975. №7. С.11-16.
5. Добровольский В. В. География микроэлементов. Глобальное рассеяние // М.: Мысль, 1983. 272с.
6. Жаксылыкова А. К., Алмабаев Ы. А., Идрисов А. А., Ткаченко Н.Л. Морфофункциональные нарушения в гепатоцитах крыс при хроническом отравлении солями кадмия // Вестник КазНМУ им. С.Ж. Асфендиярова. 2014. №2(3). С.14-17.
7. Марупов А. М., Лужников Е. Ж., Гольдфарб Ю. С. Эндотоксикоз при острых экзогенных отравлениях (обзор литературы) // Токсикологический вестник. 2004. №2. С.28.
8. Немывако Е.Е., Доровских В.А., Коршунова Н.В., Шаповаленко Н.С. Влияние пищевых добавок из биологически активного сырья на морфологическую организацию миокарда и печени крыс в условиях теплового стресса // Морфология. 2011. Т.140. №5. С.103.
9. Палаичева В. Один дома // Эксперт Казахстана. 2006. № 44.(100). С.49-53.
10. Попп Е. А. Морфофункциональная характеристика печени матери при эндогенной интоксикации и протекции цеолитами // Морфология, лимфология, клиника (Сборник научных работ посвященных 80-летию академика Ю.И.Бородина). Новосибирск, 2009. С.294-296.
11. Работникова Л. В. К сравнительной оценке значимости путей введения ядов в организм в условиях токсического эксперимента // Актуальные проблемы токсикологии. Л.,1970. С.180-185.
12. Толоконцев Н.А., Филов В.А. Основы общей промышленной токсикологии // Л.: Медицина, 1976. 304с.
13. Шкрупий В. А. Количественное исследование ультраструктурной организации гепатоцитов интактных мышей в условиях острого отравления CCL4 // Бюллетень экспериментальной биологии, 1973. №2. С.110-113.
14. Jpek J. Yilmaz, Kabay N., Yuksel M., Kizmizisakal O., Bryjak M. Removal of boron from Balcova geothermal water by ion exchange microfiltration hybrid process // Desalination. 2009. V.241. P.167-173.
15. Iwamasa T., Ninomiya N., Hanvada T., Fukuda S. Immunohistochemical localization of Acid a-glucosidase in rat liver // J. Histochem. Cytochem. 1982. Vol.30. №4. P.378-384.
16. Gumicio J., Miller D. Liver cell heterogeneity // The liver biology and pathology. New York, 1982. P.647-661.
17. Miller L., Lanolli C., Gumucio J. Quantitave morphology of the sinusoids of the Hepatic acinus. Quantiment analysis of rat liver // Gastroenterlogy, 1979. Vol.76. P.965-969.
18. Novikoff P. Intracellular organells and lipoprotein metabolism in normal and futty livers // The liver: biology and pathology, New York, 1982. P.143-168.
19. Parschova H., Mistova E., Jelinek L., Kabay N., Kauppinen P. Comparison of several polymetic sorbents for selective boron removal from reverse osmosis permete // React. Funct. Polym. 2007. V.67. №12. P.1622-1627.
20. Pigkal E., Maurice M., Feldmann G. Immunoperoxidase localization of albumen and fibrinogen in rat liver fixed by perfusion of immersion: effect of saponin on the infracellular penerution of labeled antilodies // J. Histochem. Cytochem. 1982. Vol.30. №10. P.1004-1014.
21. Taniguchi M., Fusaoka Y., Nishikowa T., Kurihara M. Boron removal in RO seawater desalination // Desalination. 2004. V.167. P.419-426.
References:
1. Afanas'ev Yu. I. Yurina N. A. Pechen'. Gistologiya, tsitologiya, embriologiya (uchebnik) [Liver. Histology, Cytology, embryology (textbook)]. Moskva, 2012. pp.595-606.
2. Beketova T. P., Sekamova S. M. K voprosu strukturnoy geterogennosti gepatotsitov [On the question of structural heterogeneity of hepatocytes]. III Vsesoyuznaya Konferentsiya po patologii kletki. (Tez. dokl) [III All-Union Conference on cell pathology. (Proc. Rep)]. 1982. pp.101-102.
3. Borisov A. I. Gigienicheskie aspekty okhrany okruzhayushchey sredy [Hygienic aspects of environment protection]. Gigiena i sanitariya [Hygiene and sanitation]. 1976. №1. pp.11-16.
4. Verbitskaya G. V. Aktual'nye voprosy gigieny naselennykh mest [Actual questions of hygiene localities]. Gigiena i sanitariya [Hygiene and sanitation]. M., 1975. №7. pp.11-16.
5. Dobrovol'skiy V. V. Geografiya mikroelementov [Geography micronutrients]. Global'noe rasseyanie [Global scattering]. M.:Mysl', 1983. 272p.
6. Zhaksylykova A. K., Almabaev Y. A., Idrisov A. A., Tkachenko N. L. Morfofunktsional'nye narusheniya v gepatotsitakh krys pri khroniches-kom otravlenii solyami kadmiya [Morphological and functional abnormalities in rat hepatocytes in chronic poisoning with salts of cadmium]. Vestnik KazNMU [Bulletin KazNMU]. Almaty, 2014. №2(3). pp.14-17.
7. Marupov A. M., Luzhnikov E. Zh., Gol'dfarb Yu. S. Endotoksikoz pri ostrykh ekzogennykh otravleniyakh (obzor literatury) [Endotoxemia in acute exogenous poisoning (review)]. Toksikologicheskiy vestnik [Poison Gazette]. 2004. №2. pp. 28.
8. Nemyvako E. E., Dorovskikh V. A., Korshunova N. V., Shapovalenko N. S. Vliyanie pishchevykh dobavok iz biologicheski aktivnogo syr'ya na morfologicheskuyu organizatsiyu miokarda i pecheni krys v usloviyakh teplovogo stressa [Effect of supplementation of the active materials on the morphological organization of the myocardium and liver of rats under conditions of heat stress]. Morfologiya [Morphology]. 2011. T.140. №5. pp.103.
9. Palaicheva V. Odin doma [Alone at home]. Ekspert Kazakhstana [Expert Kazakhstan]. 2006. № 44. (100). pp.49-53.
10. Popp E. A. Morfofunktsional'naya kharakteristika pecheni materi pri endogennoy intoksikatsii i protektsii tseolitami [Morphofunctional characteristic of liver mother at endogenous intoxication and patronage zeolites]. Morfologiya, limfologiya, klinika (Sbornik nauchnykh rabot posvyashchennykh 80-letiyu akademika Yu.I.Borodin) [Morphology, lymphology clinic (Collection of scientific works dedicated to the 80th anniversary of academician Yu.I.Borodin)]. Novosibirsk, 2009. pp.294-296.
11. Rabotnikova L. V. K sravnitel'noy otsenke znachimosti putey vvedeniya yadov v organizm v usloviyakh toksicheskogo eksperimenta [For comparative evaluation of the significance of routes of administration of poisons into the body in a toxic experiment]. Aktual'nye problemy toksikologii [Actual problems of toxicology]. L.,1970. pp.180-185.
12. Tolokontsev N. A., Filov V. A. Osnovy obshchey promyshlennoy toksikologii [Fundamentals of general industrial toxicology]. L.: Meditsina [Medicine], 1976. 304p.
13. Shkrupiy V. A. Kolichestvennoe issledovanie ul'trastrukturnoy organizatsii gepatotsitov intaktnykh myshey v usloviyakh ostrogo otravleniya CCL4 [Quantitative study of the ultrastructural organization of hepatocytes intact mice in acute poisoning CCL4]. Byulleten' eksperimental'noy biologii [Bulletin of Experimental Biology]. 1973. №2. pp.110-113.
14. Jpek J. Yilmaz, Kabay N., Yuksel M., Kizmizisakal O., Bryjak M. Removal of boron from Balcova geothermal water by ion exchange microfiltration hybrid process. Desalination. 2009. V.241. pp.167-173.
15. Iwamasa T., Ninomiya N., Hanvada T., Fukuda S. Immunohistochemical localization of Acid a-glucosidase in rat liver. J. Histochem. Cytochem. 1982. Vol.30. №4. pp.378-384.
16. Gumicio J., Miller D. Liver cell heterogeneity. The liver biology and pathology. New York, 1982. pp.647-661.
17. Miller L., Lanolli C., Gumucio J. Quantitave morphology of the sinusoids of the Hepatic acinus. Quantiment analysis of rat liver. Gastroenterlogy. 1979. Vol.76. pp.965-969.
18. Novikoff P. Intracellular organells and lipoprotein metabolism in normal and futty livers. The liver: biology and pathology. New York, 1982. pp.143-168.
19. Parschova H., Mistova E., Jelinek L., Kabay N., Kauppinen P. Comparison of several polymetic sorbents for selective boron removal from reverse osmosis permete. React.Funct. Polym. 2007. V.67. №12. pp.1622-1627.
20. Pigkal E., Maurice M., Feldmann G. Immunoperoxidase localization of albumen and fibrinogen in rat liver fixed by perfusion of immersion: effect of saponin on the infracellular penerution of labeled antilodies. J. Histochem. Cytochem. 1982. Vol.30. №10. pp.1004-1014.
21. Taniguchi M., Fusaoka Y., Nishikowa T., Kurihara M. Boron removal in RO seawater desalination. Desalination. 2004. V.167. pp.419-426.
1. Афанасьев Ю. И. Юрина Н. А. Печень // Гистология, цитология, эмбриология (учебник). Москва, 2012. С.595-606.
2. Бекетова Т. П., Секамова С. М. К вопросу структурной гетерогенности гепатоцитов // III Всесоюзная Конференция по патологии клетки. (Тез. докл). 1982. С.101-102.
3. Борисов А. И. Гигиенические аспекты охраны окружающей среды // Гигиена и санитария. 1976. №1. С.11-16.
4. Вербицкая Г. В. Актуальные вопросы гигиены населенных мест // Гигиена и санитария. М., 1975. №7. С.11-16.
5. Добровольский В. В. География микроэлементов. Глобальное рассеяние // М.: Мысль, 1983. 272с.
6. Жаксылыкова А. К., Алмабаев Ы. А., Идрисов А. А., Ткаченко Н.Л. Морфофункциональные нарушения в гепатоцитах крыс при хроническом отравлении солями кадмия // Вестник КазНМУ им. С.Ж. Асфендиярова. 2014. №2(3). С.14-17.
7. Марупов А. М., Лужников Е. Ж., Гольдфарб Ю. С. Эндотоксикоз при острых экзогенных отравлениях (обзор литературы) // Токсикологический вестник. 2004. №2. С.28.
8. Немывако Е.Е., Доровских В.А., Коршунова Н.В., Шаповаленко Н.С. Влияние пищевых добавок из биологически активного сырья на морфологическую организацию миокарда и печени крыс в условиях теплового стресса // Морфология. 2011. Т.140. №5. С.103.
9. Палаичева В. Один дома // Эксперт Казахстана. 2006. № 44.(100). С.49-53.
10. Попп Е. А. Морфофункциональная характеристика печени матери при эндогенной интоксикации и протекции цеолитами // Морфология, лимфология, клиника (Сборник научных работ посвященных 80-летию академика Ю.И.Бородина). Новосибирск, 2009. С.294-296.
11. Работникова Л. В. К сравнительной оценке значимости путей введения ядов в организм в условиях токсического эксперимента // Актуальные проблемы токсикологии. Л.,1970. С.180-185.
12. Толоконцев Н.А., Филов В.А. Основы общей промышленной токсикологии // Л.: Медицина, 1976. 304с.
13. Шкрупий В. А. Количественное исследование ультраструктурной организации гепатоцитов интактных мышей в условиях острого отравления CCL4 // Бюллетень экспериментальной биологии, 1973. №2. С.110-113.
14. Jpek J. Yilmaz, Kabay N., Yuksel M., Kizmizisakal O., Bryjak M. Removal of boron from Balcova geothermal water by ion exchange microfiltration hybrid process // Desalination. 2009. V.241. P.167-173.
15. Iwamasa T., Ninomiya N., Hanvada T., Fukuda S. Immunohistochemical localization of Acid a-glucosidase in rat liver // J. Histochem. Cytochem. 1982. Vol.30. №4. P.378-384.
16. Gumicio J., Miller D. Liver cell heterogeneity // The liver biology and pathology. New York, 1982. P.647-661.
17. Miller L., Lanolli C., Gumucio J. Quantitave morphology of the sinusoids of the Hepatic acinus. Quantiment analysis of rat liver // Gastroenterlogy, 1979. Vol.76. P.965-969.
18. Novikoff P. Intracellular organells and lipoprotein metabolism in normal and futty livers // The liver: biology and pathology, New York, 1982. P.143-168.
19. Parschova H., Mistova E., Jelinek L., Kabay N., Kauppinen P. Comparison of several polymetic sorbents for selective boron removal from reverse osmosis permete // React. Funct. Polym. 2007. V.67. №12. P.1622-1627.
20. Pigkal E., Maurice M., Feldmann G. Immunoperoxidase localization of albumen and fibrinogen in rat liver fixed by perfusion of immersion: effect of saponin on the infracellular penerution of labeled antilodies // J. Histochem. Cytochem. 1982. Vol.30. №10. P.1004-1014.
21. Taniguchi M., Fusaoka Y., Nishikowa T., Kurihara M. Boron removal in RO seawater desalination // Desalination. 2004. V.167. P.419-426.
References:
1. Afanas'ev Yu. I. Yurina N. A. Pechen'. Gistologiya, tsitologiya, embriologiya (uchebnik) [Liver. Histology, Cytology, embryology (textbook)]. Moskva, 2012. pp.595-606.
2. Beketova T. P., Sekamova S. M. K voprosu strukturnoy geterogennosti gepatotsitov [On the question of structural heterogeneity of hepatocytes]. III Vsesoyuznaya Konferentsiya po patologii kletki. (Tez. dokl) [III All-Union Conference on cell pathology. (Proc. Rep)]. 1982. pp.101-102.
3. Borisov A. I. Gigienicheskie aspekty okhrany okruzhayushchey sredy [Hygienic aspects of environment protection]. Gigiena i sanitariya [Hygiene and sanitation]. 1976. №1. pp.11-16.
4. Verbitskaya G. V. Aktual'nye voprosy gigieny naselennykh mest [Actual questions of hygiene localities]. Gigiena i sanitariya [Hygiene and sanitation]. M., 1975. №7. pp.11-16.
5. Dobrovol'skiy V. V. Geografiya mikroelementov [Geography micronutrients]. Global'noe rasseyanie [Global scattering]. M.:Mysl', 1983. 272p.
6. Zhaksylykova A. K., Almabaev Y. A., Idrisov A. A., Tkachenko N. L. Morfofunktsional'nye narusheniya v gepatotsitakh krys pri khroniches-kom otravlenii solyami kadmiya [Morphological and functional abnormalities in rat hepatocytes in chronic poisoning with salts of cadmium]. Vestnik KazNMU [Bulletin KazNMU]. Almaty, 2014. №2(3). pp.14-17.
7. Marupov A. M., Luzhnikov E. Zh., Gol'dfarb Yu. S. Endotoksikoz pri ostrykh ekzogennykh otravleniyakh (obzor literatury) [Endotoxemia in acute exogenous poisoning (review)]. Toksikologicheskiy vestnik [Poison Gazette]. 2004. №2. pp. 28.
8. Nemyvako E. E., Dorovskikh V. A., Korshunova N. V., Shapovalenko N. S. Vliyanie pishchevykh dobavok iz biologicheski aktivnogo syr'ya na morfologicheskuyu organizatsiyu miokarda i pecheni krys v usloviyakh teplovogo stressa [Effect of supplementation of the active materials on the morphological organization of the myocardium and liver of rats under conditions of heat stress]. Morfologiya [Morphology]. 2011. T.140. №5. pp.103.
9. Palaicheva V. Odin doma [Alone at home]. Ekspert Kazakhstana [Expert Kazakhstan]. 2006. № 44. (100). pp.49-53.
10. Popp E. A. Morfofunktsional'naya kharakteristika pecheni materi pri endogennoy intoksikatsii i protektsii tseolitami [Morphofunctional characteristic of liver mother at endogenous intoxication and patronage zeolites]. Morfologiya, limfologiya, klinika (Sbornik nauchnykh rabot posvyashchennykh 80-letiyu akademika Yu.I.Borodin) [Morphology, lymphology clinic (Collection of scientific works dedicated to the 80th anniversary of academician Yu.I.Borodin)]. Novosibirsk, 2009. pp.294-296.
11. Rabotnikova L. V. K sravnitel'noy otsenke znachimosti putey vvedeniya yadov v organizm v usloviyakh toksicheskogo eksperimenta [For comparative evaluation of the significance of routes of administration of poisons into the body in a toxic experiment]. Aktual'nye problemy toksikologii [Actual problems of toxicology]. L.,1970. pp.180-185.
12. Tolokontsev N. A., Filov V. A. Osnovy obshchey promyshlennoy toksikologii [Fundamentals of general industrial toxicology]. L.: Meditsina [Medicine], 1976. 304p.
13. Shkrupiy V. A. Kolichestvennoe issledovanie ul'trastrukturnoy organizatsii gepatotsitov intaktnykh myshey v usloviyakh ostrogo otravleniya CCL4 [Quantitative study of the ultrastructural organization of hepatocytes intact mice in acute poisoning CCL4]. Byulleten' eksperimental'noy biologii [Bulletin of Experimental Biology]. 1973. №2. pp.110-113.
14. Jpek J. Yilmaz, Kabay N., Yuksel M., Kizmizisakal O., Bryjak M. Removal of boron from Balcova geothermal water by ion exchange microfiltration hybrid process. Desalination. 2009. V.241. pp.167-173.
15. Iwamasa T., Ninomiya N., Hanvada T., Fukuda S. Immunohistochemical localization of Acid a-glucosidase in rat liver. J. Histochem. Cytochem. 1982. Vol.30. №4. pp.378-384.
16. Gumicio J., Miller D. Liver cell heterogeneity. The liver biology and pathology. New York, 1982. pp.647-661.
17. Miller L., Lanolli C., Gumucio J. Quantitave morphology of the sinusoids of the Hepatic acinus. Quantiment analysis of rat liver. Gastroenterlogy. 1979. Vol.76. pp.965-969.
18. Novikoff P. Intracellular organells and lipoprotein metabolism in normal and futty livers. The liver: biology and pathology. New York, 1982. pp.143-168.
19. Parschova H., Mistova E., Jelinek L., Kabay N., Kauppinen P. Comparison of several polymetic sorbents for selective boron removal from reverse osmosis permete. React.Funct. Polym. 2007. V.67. №12. pp.1622-1627.
20. Pigkal E., Maurice M., Feldmann G. Immunoperoxidase localization of albumen and fibrinogen in rat liver fixed by perfusion of immersion: effect of saponin on the infracellular penerution of labeled antilodies. J. Histochem. Cytochem. 1982. Vol.30. №10. pp.1004-1014.
21. Taniguchi M., Fusaoka Y., Nishikowa T., Kurihara M. Boron removal in RO seawater desalination. Desalination. 2004. V.167. pp.419-426.
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Bibliography link
Berdalinova A. K., Umbetov T. Zh., Komekbai Zh. E. Morphology of the in chronic intoxication of the organism with sodium tetraborate. Nauka i Zdravoohranenie [Science & Healthcare]. 2015, 3, pp. 89-97.
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