COMPARATIVE EVALUATION OF TYPING METHODS FOR E.COLI - MLST AND SEROTYPING
Background and Objectives: Subspecies typing methods allow to carry out of genetic relationship (clonality) analysis between strains within species and to establish a possible epidemiological link between the source of infection, the factors of transmission. Identify and compare the results of serological and multilocus sequencing typing data based on E.coli genomes.
Material and methods: Determination of serotypes and MLST types of E.coli have been done based on genome-wide data using a web resource Center of Genomic Epidemiology (www.genomicepidemiology.org). Discriminatory power and concordance of the results was calculated using Rand and adj.Wallace indeces.
Results and Discussion: Research based on the genome-wide data E.coli with determination MLST and serotypes demonstrated the same level of discriminating ability (95-96%). Concordance between the results obtained by the two methods was 83.9% (95% CI 80,2-86,5). The predictive ability for the MLST-based O:H serotypes was 76%, for serotypes using MLST data was 92.2%
Conclusion: While resolution power of serotyping and MLST analysis were at same level (95-96%), the concordance of the results was was 84%. There was very good conformity between some MLST types and serotypes, at the same time, other MLST types were characterized with different serotypes.
D. B. Babenko1, A. A. Turmukhambetova1, N. Ye. Aukenov2
1 Karaganda State Medical University, Karaganda, Kazakstan;
2 Semey State Medical University, Semey, Kazakstan.
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Babenko D. B., Turmukhambetova A. A., Aukenov N. Ye. Comparative evaluation of typing methods for E.coli - MLST and serotyping. Nauka i Zdravoohranenie [Science & Healthcare]. 2015, 6, pp. 77-82.Related publications:
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