HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF PLASMA LEVELS OF VITAMINS GROUP «B» IN METABOLIC SYNDROME
Background: Examining blood plasma for B vitamins can be pivotal in detecting metabolic syndrome (MetS), a group of risk factors that raise the likelihood of acquiring cardiovascular disease and type 2 diabetes. B vitamins, namely thiamine (B1), riboflavin (B2), pantothenic acid (B5), pyridoxine (B6), biotin (B7), folate (B9), and cobalamin (B12), are critical for multiple metabolic functions in the body, such as generating energy, synthesizing DNA, and maintaining nerve function. Inadequate levels or uneven ratios of these B vitamins can contribute to the onset and advancement of MetS.
Objective: To detect variations in the levels of B vitamins (В1, В2, В5, B6, В7, B9, and B12) in the blood plasma of patients with MetS by High-Performance Liquid Chromatography (HPLC).
Materials and methods: Fifteen patients who have been diagnosed with MetS and meet the relevant criteria were selected, and samples of their plasma were collected to determine the levels of vitamin B1, B2, B5, B6, B7, B9, and B12 by HPLC. Additionally, plasma samples were also collected and analyzed from fifteen apparently healthy individuals. The samples were evaluated for their concentration levels of vitamins B i group n patients with MetS and apparently healthy individuals.
Results and discussion: The study findings demonstrated a significant decrease of plasma levels of vitamins B1 and B9 in patients with MetS compared with healthy individuals, whereas plasma levels of vitamin B12 were twice as high as control group. To evaluate the modified method and customize the previously developed assay technique to the specific needs of blood testing, a relatively larger sample size is required to confirm these findings.
Alma Nurtazina1, https://orcid.org/0000-0002-9737-4003
Maxat Toishimanov2, https://orcid.org/0000-0002-6070-4574
Ivan Voitsekhovskiy3, https://orcid.org/0000-0001-6920-2776
Daulet Dautov4, https://orcid.org/0000-0003-0838-6983
1 NJSC «Semey medical university», Semey, Republic of Kazakhstan;
2 The Kazakhstan-Japan Innovation Center KazNARU, Almaty, Republic of Kazakhstan;
3 Al Farabi Kazakh National University, Almaty, Republic of Kazakhstan;
4 NJSC “Asfendiyarov Kazakh National Medical University”, Almaty, Republic of Kazakhstan.
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Nurtazina A., Toishimanov M., Voitsekhovskiy I., Dautov D. High Performance Liquid Chromatography Analysis of Plasma Levels of Vitamins Group «B» in Metabolic Syndrome // Nauka i Zdravookhranenie [Science & Healthcare]. 2023, (Vol.25) 2, pp. 79-83. doi 10.34689/SH.2023.25.2.011Related publications:
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