CHANGES OF PLASMA FREE AMINO ACIDS IN METABOLIC SYNDROME
Background: Studies have been going on for a while now to investigate the link between plasma free amino acids (PFAA) and metabolic syndrome, and the results so far have been quite promising. However, these analyses aren't commonly used for early clinical diagnosis of metabolic syndrome, partly because mass spectrometry can be pretty expensive. That's why we decided to look into and characterize the PFAA profile of blood plasma in metabolic syndrome patients using a more affordable alternative to MS\MS or GC-MS methods: high performance liquid chromatography (HPLC).
Objective: How can we describe the changes in the levels of free amino acids found in the bloodstream of individuals diagnosed with metabolic syndrome using high-performance liquid chromatography (HPLC)?
Materials and methods: We sampled thirty patients who met the diagnostic criteria for metabolic syndrome from their plasma for PFAA determination using HPLC. Additionally, we took plasma samples from fifteen confirmed healthy individuals and analyzed them using HPLC. We measured the concentrations in the samples and analyzed the relationship between the profiles of free amino acids in the plasma.
Results and discussion: The results show a significant difference in plasma concentrations of alanine, cysteine, tyrosine, valine, cystine and isoleucine between the two groups. The HPLC method, when utilized under specific conditions, can be employed for the quantitative measurement of amino acids in the blood.
Alma Nurtazina1, https://orcid.org/0000-0002-9737-4003
Ivan Voitsekhovskiy2, https://orcid.org/0000-0001-6920-2776
Maxat Toishimanov3, https://orcid.org/0000-0002-6070-4574
Daulet Dautov4, https://orcid.org/0000-0003-0838-6983
1 NJSC «Semey medical university», Semey, Republic of Kazakhstan;
2 Al Farabi Kazakh National University, Almaty, Republic of Kazakhstan;
3 The Kazakhstan-Japan Innovation Center KazNARU, Almaty, Republic of Kazakhstan;
4 NJSC “Asfendiyarov Kazakh National Medical University”, Almaty, Republic of Kazakhstan.
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Nurtazina A., Voitsekhovskiy I., Toishimanov M., Dautov D. Сhanges of plasma free amino acids in metabolic syndrome // Nauka i Zdravookhranenie [Science & Healthcare]. 2023, (Vol.25) 2, pp. 84-89. doi 10.34689/SH.2023.25.2.012Related publications:
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