CLINICAL AND MORPHOLOGICAL DETERMINATION OF THE OPTIMAL CANNULA DIAMETER FOR LIPOSUCTION IN RABBITS: EXPERIMENTAL STUDY
Relevance: Determining the diameter of the cannula most suitable for liposuction is of great importance from the point of view of both the quality and composition of the resulting material and the convenience of practical use. Previously published studies have shown that the use of cannulas with a diameter of 2.5 mm or more contributes to the preservation of a more natural structure of adipose tissue compared to cannulas 1 and 2 mm. There is a direct relationship between the percentage of connective-woven fibers in the lipoaspirate and the diameter of the cannula. The lack of clear criteria of choosing a cannula for lipoaspiration is one of the limitations for obtaining generally accepted lipoaspiration protocols with subsequent use of adipose tissue.
Objective: Clinical and histomorphometric determination of the optimal cannula diameter for inguinal lipoaspirate sampling in an experimental study on rabbits.
Materials and methods: Study design – experiment. The study was conducted on rabbits, the animals were divided into 4 groups. All rabbits underwent liposuction using the Columan method. Cannulas with a diameter of 1.0mm, 2.0mm, 3.0mm, 4.0mm were used. During the operation, the number of reciprocating movements per 1 minute and the number of cases of blockage of the stables were counted. Next, macroscopic and histomorphometric assessments of the fat lipoaspirate were carried out.
Results: The average volume of the obtained material in the groups with medium and large cannula diameter was 2.5 cm3 and 2.8 cm3, and in the groups with minimum and small cannula diameter 0.5 cm3 and 1.6 cm3. In all groups, the average relative amount of adipose tissue was 90-95%, and the average relative amount of fibrous tissue was less than 10%.
In groups with a minimal, small cannula diameter, the average size of stroma fragments was less than 1mm, and in the group with a large cannula diameter in 37.5% of cases, the average size of fragments was 1-1.5 mm, and 25% of cases exceeded 1.5 mm. Artifacts in all groups occupied no more than 10% of the area, less than 2% of artifacts they were occupied in groups with medium and large diameter cannula in 50% and 80% of cases, respectively.
Conclusions: Histomorphometric analysis and evaluation according to a system of subjective criteria showed that the choice of a cannula with a diameter of 3 mm makes it possible to obtain a lipoaspirate with evenly distributed fibrous tissue of optimal size.
Ilona L. Pak1, https://orcid.org/0000-0002-4388-1982
Meirbek S. Askarov1, https://orcid.org/0000-0002-5978-2989
Dmitriy A. Klyuev1, https://orcid.org/0000-0003-2012-2227
Yevgeniy K. Kamyshansky1, https://orcid.org/0000-0001-8975-3612
1 Karaganda Medical University, Karaganda, Republic of Kazakhstan.
1. Приказ Министра здравоохранения Республики Казахстан от 11.12.2020 г. № ҚР ДСМ-248/2020. «Об утверждении правил проведения клинических исследований лекарственных средств и медицинских изделий, клинико-лабораторных испытаний медицинских изделий для диагностики вне живого организма (in vitro) и требования к клиническим базам и оказания государственной услуги Выдача разрешения на проведение клинического исследования и (или) испытания фармакологических и лекарственных средств, медицинских изделий» Зарегистрирован в Министерстве юстиции Республики Казахстан 14 декабря 2020 года № 21772. https://adilet.zan.kz/rus/docs/V2000021772/info, (Дата обращения: 09.12.2022)
2. Стандарт надлежащей лабораторной практики (GLP). Приказ Министра здравоохранения и социального развития Республики Казахстан от 27 мая 2015 года № 392 «Об утверждении надлежащих фармацевтических практик» Зарегистрирован в Министерстве юстиции Республики Казахстан 30 июня 2015 года № 11506. https://adilet.zan.kz/rus/docs/V1500011506, (Дата обращения: 28.01.2023)
3. Beck D.O., Davis K., Rohrich R.J. Enhancing lipoaspirate efficiency by altering liposuction cannula design // Plast Reconstr Surg Glob Open. 2014. №2. Р.222.
4. Bluguermann C., Wu L., Petrigliano F., McAllister D., Miriuka S., Evseenko D.A. Novel aspects of parenchymal-mesenchymal interactions: From cell types to molecules and beyond // Cell Biochem Funct. 2013. №31. Р.271–280.
5. Campbell G.-L., Laudenslager N., Newman J. The effect of mechanical stress on adipocyte morphology and metabolism // Am. J. Cosmet. Surg. 1987. №4. P. 89-94.
6. Canizares O., Thomson J.E., Allen R.J. et al. The effect of processing technique on fat graft survival. Plast Reconstr Surg. 2017. №140. Р.933.
7. Del Vecchio D., Rohrich R.J. A classification of clinical fat grafting: different problems, different solutions // Plast Reconstr Surg. 2012. №130. Р.511-22.
8. Erdim M., Tezel E., Numanoglu A., et al. The effects of the size of liposuction cannula on adipocyte survival and the optimum temperature for fat graft storage: an experimental study // J Plast Reconstr Aesthet Surg. 2009. №62. Р.1210.
9. European Convention for the Protection of Vertebrate Animals used for. Experimental and Other Scientific Purposes*. Strasbourg, 18.III.1986. https://rm.coe.int/168007a67b, (accessed: 14.02.2022)
10. Gause T.M., Kling R.E., Sivak W.N. et al. Particle size in fat graft retention: A review on the impact of harvesting technique in lipofilling surgical outcomes. Adipocyte. 2014. №3. Р.273–279
11. Kato H., Mineda K., Eto H., Doi K., Kuno S., Kinoshita K., Kanayama K., Yoshimura K. Degeneration, regeneration, and cicatrization after fat grafting: dynamic total tissue remodeling during the first 3 months // Plast Reconstr Surg. 2014. №133. Р.303-13.
12. Kirkham J.C., Lee J.H., Medina M.A., 3rd, et al. The impact of liposuction cannula size on adipocyte viability // Ann Plast Surg. 2012. 69. p.479.
13. Ozsoy Z., Kul Z., Bilir A. The role of cannula diameter in improved adipocyte viability: a quantitative analysis // Aesthet Surg J. 2006. №26(3). P.287-9.
References: [1-2]
1. Order of the Minister of Health of the Republic of Kazakhstan dated December 11, 2020 No. KR DSM-248/2020."On approval of the Rules for Conducting Clinical Trials of Medicines and Medical Devices, clinical and laboratory tests of Medical Devices for diagnostics outside a Living Organism (in vitro) and Requirements for Clinical Databases and the Provision of Public Services for the Issuance of Permits for Conducting clinical trials and (or) testing of pharmacological and Medicinal Products, medical Devices" [Order of the Minister of Health of the Republic of Kazakhstan dated December 11, 2020 No. ҚР ДСМ-248/2020. "On approval of the rules for conducting clinical trials of medicines and medical devices, clinical and laboratory tests of medical devices for diagnostics outside a living organism (in vitro) and requirements for clinical facilities and the provision of public services Issuance of a permit for conducting clinical trials and (or) testing of pharmacological and medicinal products, medical devices" Registered with the Ministry of Justice of the Republic of Kazakhstan on December 14, 2020 No. 21772. registered with the Ministry of Justice Of the Republic of Kazakhstan on December 14, 2020 No. 21772. https://adilet.zan.kz/rus/docs/V2000021772/info, (accessed: 04.02.2022)] [in Russian]
2. Standart nadlezhashhej laboratornoj praktiki (GLP). Prikaz Ministra zdravoohranenija i social'nogo razvitija Respubliki Kazahstan ot 27 maja 2015 goda № 392 «Ob utverzhdenii nadlezhashhih farmacevticheskih praktik» Zaregistrirovan v Ministerstve justicii Respubliki Kazahstan 30.06.2015 goda № 11506. [The Standard of Good Laboratory Practice (GLP). from the Order of the Minister of Health and Social Development of the Republic of Kazakhstan dated May 27, 2015 No. 392 "On approval of appropriate pharmaceutical practices" was registered with the Ministry of Justice of the Republic of Kazakhstan] on 30.06.2015 No.11506. https://adilet.zan.kz/rus/docs/V1500011506, (accessed: 28.01.2023) [in Russian]
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Pak I.L., Askarov M.S., Klyuev D.A., Kamyshansky E.K. Clinical and morphological determination of the optimal cannula diameter for liposuction in rabbits: experimental study // Nauka i Zdravookhranenie [Science & Healthcare]. 2023, (Vol.25) 3, pp. 47-54. doi 10.34689/SH.2023.25.3.006Related publications:
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