PREOPERATIVE USE OF PLATELET-RICH PLASMA FOR LIPOFILLING
Relevance: Lipofilling is becoming an increasingly popular method of reconstruction the volume of almost any part of the human body every day. However, the use of adipose tissue as a filler has its limitations in the long term in the form of a low percentage of fat graft survival as a result of fibrous replacement and apoptosis of fat cells.
Aim: the aim of this study was a comparative morphological assessment of the survival of a fat autograft in groups with a standard lipofilling protocol and with preimplantation treatment of the implantation zone with platelet-rich plasma in rabbits.
Methods: in this study, 24 male rabbits were used, who underwent subcutaneous implantation of a fat autograft in the auricle area. All cases were divided into 3 groups depending on the method of treatment with platelet-rich plasma. After 3 months (90 days) of exposure, a macroscopic and histological examination of the fat autograft was performed.
Results: in the group of preoperative treatment of the implantation zone and intraoperative autograft treatment, a statistically significant preservation of the volume and histological normal fat autograft was observed in comparison with the group without and only intraoperative treatment.
Conclusion: preimplantation use of platelet-rich plasma significantly improves the standard technique of intraoperative treatment, increases the survival of fat autograft by increasing angiogenesis with a decrease in the degree of hypoxic-ischemic, fibrotic and inflammatory damage in the implantation zone
Ilona L. Pak1, https://orcid.org/0000-0002-4388-1982
Meirbek S. Askarov1, https://orcid.org/0000-0002-5978-2989
Dmitriy A. Klyuev1, https://orcid.org/0000-0003-2012-2227
Yevgeniy K. Kamyshansky1, https://orcid.org/0000-0001-8975-3612
1 Karaganda Medical University,
Karaganda, Republic of Kazakhstan.
1. Приказ Министра здравоохранения Республики Казахстан от 11 декабря 2020 года № ҚР ДСМ-248/2020. «Об утверждении правил проведения клинических исследований лекарственных средств и медицинских изделий, клинико-лабораторных испытаний медицинских изделий для диагностики вне живого организма (in vitro) и требования к клиническим базам и оказания государственной услуги Выдача разрешения на проведение клинического исследования и (или) испытания фармакологических и лекарственных средств, медицинских изделий» Зарегистрирован в Министерстве юстиции Республики Казахстан 14 декабря 2020 года № 21772. https://adilet.zan.kz/rus/docs/V2000021772/info, (Дата обращения: 02.04.2022)
2. Стандарт надлежащей лабораторной практики (GLP). Приказ Министра здравоохранения и социального развития Республики Казахстан от 27 мая 2015 года №392 «Об утверждении надлежащих фармацевтических практик» Зарегистрирован в Министерстве юстиции Республики Казахстан 30 июня 2015 года № 11506. https://adilet.zan.kz/rus/docs/V1500011506, (Дата обращения: 19.04.2022)
3. Allgoewer A., Mayer B. Sample size estimation for pilot animal experiments by using a Markov Chain Monte Carlo approach // Altern Lab Anim, (2017). 45(2), pp. 83-90;
4. Coleman S.R. Hand rejuvenation with structural fat grafting // Plastic and Reconstructive Surgery, 2002. 110, 1731 discussion 1745.
5. Coleman S.R. Long-term survival of fat transplants: controlled demonstrations // Aesthetic Plastic Surgery, (1995). 19, p. 421
6. Coleman S.R. Structural fat grafts: the ideal filler? // Clin. Plastic Surgery (2001). 28, p. 111
7. Delay E., Garson S., Tousson G. et al. Fat injection to the breast: technique, results, and indications based on 880 procedures over 10 years // Aesthet Surg J., 2009. 29, pp. 360-376
8. DeRossi R., Coelho A.C., Mello G.S. et al. Effects of platelet-rich plasma gel on skin healing in surgical wound in horses // Acta Cir Bras. 2009. Jul-Aug, 24(4):276–281.
9. El-Sharkawy H., Kantarci A., Deady J. et al. Platelet-rich plasma: growth factors and pro-and anti-inflammatory properties // J Periodontol. 2007. 78: 661- 669.
10. Eto H., Kato H., Suga H. et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes // Plast Reconstr Surg. 2012. May,129(5):1081–1092
11. European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes*. Strasbourg, 18.III.1986. https://rm.coe.int/168007a67b, (Дата обращения: 02.02.2023)
12. Findikcioglu F., Findikcioglu K., Yavuzer R., et al. Effect of intraoperative platelet-rich plasma and fibrin glue application on skin flap survival // J Craniofac Surg. 2012. Sep, 23(5):1513–1517.
13. Hersant B., Bouhassira J., SidAhmed-Mezi M., Vidal L., Keophiphath M., Chheangsun B., Niddam J., Bosc R., Le Nezet A., Meningaud J-P., Rodriguez A.M. // An International Journal of Surgical Reconstruction, Should platelet-rich plasma be activated in fat grafts? An animal study, 2018. 71(5), pp. 681-690.
14. Jin R., Zhang L., Zhang Y.G. Does platelet-rich plasma enhance the survival of grafted fat? An update review // Int J Clin Exp Med. 2013. 6, pp. 252-258
15. Kato H., Mineda K., Eto H., et al. Degeneration, regeneration, and cicatrization after fat grafting: dynamic total tissue remodeling during the first 3 months // Plast Reconstr Surg, 2014. 133, pp.303e-313е
16. Liao H.T. Marra K.G., Rubin J.P. Application of platelet-rich plasma and platelet-rich fibrin in fat grafting: basic science and literature review // 2014. Tissue Eng Part B Rev. 20: 267-276
17. Li Y., Mou S., Xiao P. et al. Delayed two steps PRP injection strategy for the improvement of fat graft survival with superior angiogenesis // Sci Rep (2020). 10, 5231. https://doi.org/10.1038/s41598-020-61891-6
18. Minn K.W., Min K.H., Chang H. et al. Effects of fat preparation methods on the viabilities of autologous fat grafts // Aesthetic Plast Surg., 2010, vol. 34 (pg. 626-631.
19. Missana M.C. et al. Autologous fat transfer in reconstructive breast surgery: indications, technique and results // Eur J Surg Oncol, 2007. 33, pp. 685-690
20. Modarressi A. Platelet rich plasma (PRP) improves fat grafting outcomes // World J Plast Surg, 2013. 2, pp. 6-13.
21. Nakamura S., Ishihara M., Takikawa M., et al. Platelet-rich plasma (PRP) promotes survival of fat-grafts in rats // Ann Plast Surg, 2010. 65, pp.101-106
22. Nishimura T., Hashimoto H., Nakanishi I., Furukawa M. Microvascular angiogenesis and apoptosis in the survival of free fat grafts // Laryngoscope. 2000. Aug, 110(8):1333-8. doi: 10.1097/00005537-200008000-00021. PMID: 10942136.
23. Oh D.S., Cheon Y.W., Jeon Y.R. et al. Activated platelet-rich plasma improves fat graft survival in nude mice: a pilot study // Dermatol Surg. 2011. 37: 619-625
24. Pires Fraga M.F., Nishio R.T., Ishikawa R.S. et al. Increased survival of free fat grafts with platelet-rich plasma in rabbits // J Plast Reconstr Aesthet Surg. 2010. 63: e818-e822
25. Por Y.C., Yeow V.K., Louri N. et al. Platelet-rich plasma has no effect on increasing free fat graft survival in the nude mouse // J Plast Reconstr Aesthet Surg. 2009. 62: 1030-1034
26. Rodriguez-Flores J., Palomar-Gallego M.A., Enguita-Valls A.B. et al. Influence of platelet-rich plasma on the histologic characteristics of the autologous fat graft to the upper lip of rabbits // Aesthetic Plast Surg. 2011. 35: 480-486
27. Serra-Mestre J.M., Serra-Renom J.M., Martinez L., et al. Platelet-rich plasma mixed-fat grafting: a reasonable prosurvival strategy for fat grafts // Aesthetic Plast Surg, (2014). 38, pp.1041-1049
28. Sönmez T.T., Vinogradov A., Zor F. et al.The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice // Biomaterials. 2013. Ap, 34(11):2674–2682.
29. Takikawa M., Sumi Y., Ishihara M. et al. PRP&F/P MPs improved survival of dorsal paired pedicle skin flaps in rats // J Surg Res. 2011. Sep, 170(1):e189–96.
30. Thanik V.D., Chang C.C., Lerman O.Z. et al. A murine model for studying diffusely injected human fat // Plast Reconstr Surg. 2009. 124:74e81
31. Torio-Padron N., Paul D., von Elverfeldt D., Stark G.B., Huotari A.M. Resorption rate assessment of adipose tissue-engineered constructs by intravital magnetic resonance imaging // J Plast Reconstr Aesthet Surg. 2011. Jan, 64(1):117e22
References: [1-2].
1. Prikaz Ministra zdravookhraneniya Respubliki Kazakhstan ot 11 dekabrya 2020 goda № ҚR DSM-248/2020. «Ob utverzhdenii pravil provedeniya klinicheskikh issledovanii lekarstvennykh sredstv i meditsinskikh izdelii, kliniko-laboratornykh ispytanii meditsinskikh izdelii dlya diagnostiki vne zhivogo organizma (in vitro) i trebovaniya k klinicheskim bazam i okazaniya gosudarstvennoi uslugi Vydacha razresheniya na provedenie klinicheskogo issledovaniya i (ili) ispytaniya farmakologicheskikh i lekarstvennykh sredstv, meditsinskikh izdelii» Zaregistrirovan v Ministerstve yustitsii Respubliki Kazakhstan 14 dekabrya 2020 goda № 21772 [Order of the Minister of Health of the Republic of Kazakhstan dated December 11, 2020 No. ҚР ДСМ-248/2020. "On approval of the rules for conducting clinical trials of medicines and medical devices, clinical and laboratory tests of medical devices for diagnostics outside a living organism (in vitro) and requirements for clinical facilities and the provision of public services Issuance of a permit for conducting clinical trials and (or) testing of pharmacological and medicinal products, medical devices" Registered with the Ministry of Justice of the Republic of Kazakhstan on December 14, 2020 No. 21772]. https://adilet.zan.kz/rus/docs/V2000021772/info, (accessed: 02.04.2022)
2. Standart nadlezhashchei laboratornoi praktiki (GLP). Prikaz Ministra zdravookhraneniya i sotsial'nogo razvitiya Respubliki Kazakhstan ot 27 maya 2015 goda №392 «Ob utverzhdenii nadlezhashchikh farmatsevticheskikh praktik» Zaregistrirovan v Ministerstve yustitsii Respubliki Kazakhstan 30 iyunya 2015 goda № 11506 [Good Laboratory Practice (GLP) standard. Order of the Minister of Health and Social Development of the Republic of Kazakhstan dated May 27, 2015 No. 392 “On Approval of Good Pharmaceutical Practices” Registered with the Ministry of Justice of the Republic of Kazakhstan on June 30, 2015 No. 11506]. https://adilet.zan.kz/rus/docs/V1500011506, (accessed: 19.04.2022
Number of Views: 69
Category of articles:
Original articles
Bibliography link
Pak I.L., Askarov M.S., Klyuev D.A., Kamyshansky E.K. Preoperative use of platelet-rich plasma for lipofilling // Nauka i Zdravookhranenie [Science & Healthcare]. 2023, (Vol.25) 3, pp. 55-63. doi 10.34689/SH.2023.25.3.007Related publications:
ANALYSIS OF CLINICAL AND DEMOGRAPHIC INDICATORS OF PATIENTS WITH PARKINSON'S DISEASE IN THE SOUTHERN REGION OF KAZAKHSTAN
A COMPARATIVE STUDY OF DRUG POLICY REGARDING ORPHAN DISEASES IN THE USA, THE EUROPEAN UNION, AND THE REPUBLIC OF KAZAKHSTAN
MUTATIONS IN CARDIAC ION CHANNEL GENES IN KAZAKHSTANI PATIENTS WITH LONG QT SYNDROME
GENETIC FEATURES OF CONGENITAL DYSFUNCTION OF THE ADRENAL CORTEX CAUSED BY 21-HYDROXYLASE DEFICIENCY IN PERSONS OF KAZAKH NATIONALITY
NON-MOTOR SYMPTOMS IN PARKINSON'S DISEASE USING THE EXAMPLE OF AKTOBE PATIENTS