Science & Healthcare

Introduction. Analysis of materials that characterize the human environment in the Aral Sea, has shown a sharp increase of the chemical (pesticides, fertilizers, salt components, heavy metals) pollution of drinking water, air, soil, pesticide contamination of food products of plant and animal origin, fishery products.

Aim. Еvaluate the ultrastructural changes in granulosa cells of mice under the exposure of lindane pesticide in vitro.

Methods. The experimental work carried out in the Laboratory Centre of Microscopy Department of Life, Health and Environmental Sciences University L’Aquila, Italy; 80 female mice control (10), control with solution of dimethyl sulfoxide (10), lindane 1 μM (20), lindane 10 μM (20), lindane 100 μM (20). The material of the study were ovarian granulosa cells. After preparing the samples for transmission electron microscopy, ultra-thin sections (60-80 nm) were obtained on an ultramicrotome and contrasted with salts of heavy metals with uranyl acetate and lead citrate (Sic Rome, Italy).

Results. Comparative analysis of the group showed the dependence of ultrastructural changes in the applied lindane concentration.The presence of irregularly shaped cells were observed early signs of fragmentation of the cytoplasm, which was filled with a plurality of mitochondria and fatty droplets. In the group with the highest concentration of toxicant cells appeared marginalization of chromatin remnants of apoptotic bodies and single organelles.

Conclusion. The exposure of lindane in vitro have shown a dose-dependent toxicity effect on the granulosa cells; ultrastructural changes caused granulosa cells strictly correlated on the applied dose.