EFFECT OF EXTRACT ON ACTIVITY OF ENERGY METABOLISM ENZYMES AFTER COMBINED EFFECT OF EMOTIONAL STRESS AND SUBLETHAL DOSE OF γ-RADIATION IN REMOTE PERIOD
Introduction. During adaptation process complex changes occur in metabolic pathways; functional and structural changes occur in organs and tissue. Despite the great interest of researchers to this problem, the pathogenetic mechanisms of influence of stress, especially combined effect of stressors on the metabolic processes hasn’t been studied enough. Main criterion for the stability of the adaptive behavior of the organism is to maintain energy potential of biosystems constant. Given the importance of this system, it is necessary to define its role in formation of pathological process in animals in remote period under combined effect of γ-radiation and emotional stress and their corrections.
Purpose – study effect of extract Eminium Regelii on activity of energy metabolism enzymes in immunocompetent organs in remote period under combined effects of sublethal doses of γ-radiation (6 Gy), and emotional stress.
Materials and methods. To achieve this goal, experiments were performed on 70 white male laboratory Wistar rats, weighing 240 ± 20 g, which were divided into 7 groups. Emotional stress was modeled in animals by tail suspension, and were withdrawn from the experiment after 1, 2, 3 days by partial decapitation, previously put to sleep with ether anesthetics. Prior to research, animals were exposed to radiation once with 6 Gy for 90 days on radiotherapy installation TERAGAM Сo60 («ISOTREND spol. s.r.o.», Czeck Republic)
Results. Decrease in activity of succinate dehydrogenase in spleen, adrenal glands and peripheral blood lymphocytes and in all investigated organs cytochrome oxidase’s activity has shown stressed conditions of metabolic and enzymatic processes, leading to a shortage of energy supply. Under combined effects, result was more pronounced on day 3. Research has shown positive effect of the extract in increasing enzyme activity.
Conclusion. Under effect of double factors – combination of sublethal doses of γ-radiation and emotional stress in remote period, a decline in the activity of energy metabolism enzymes occur. Administration of 2.5 ml / kg of Eminium Regelii causes an increase in enzyme activity, which was decreased as a result of combined effects.
Number of Views: 405
Category of articles:
Original articles
Bibliography link
Ильдербаева Г.О. Влияние экстракта на активность ферментов энергетического метаболизма после сочетанного действия эмоционального стресса и сублетальной дозы γ-излучения в отдаленном периоде / / Наука и Здравоохранение. 2016. №3. С. 107-118. Ilderbayeva G.O. Effect of extract on activity of energy metabolism enzymes after combined effect of emotional stress and sublethal dose of γ-radiation in remote period. Nauka i Zdravookhranenie [Science & Healthcare]. 2016, 3, pp. 107-118. Ілдербаева Г.О. Сублеталды дозадағы иондағыш сәуленің кейінгі мерзімі мен эмоциялық күйзелістің қосар әсеріндегі энергиялы алмасу ферменттері белсенділігіне сығынды әсері / / Ғылым және Денсаулық сақтау. 2016. №3. Б. 107-118.Related publications:
VALIDATION OF THE KAZAKH VERSION OF THE DEPRESSION ANXIETY STRESS SCALE (DASS-21) IN MEDICAL FACULTY STAFF SAMPLE: THE PILOT STUDY
PREDICTIVE VALUE OF PSYCHOMETRIC TESTING IN CONTEXT OF CREATING ADAPTIVE ENVIRONMENT FOR HIGHER MEDICAL EDUCATION
ASSESSMENT OF STUDENTS' AWARENESS ABOUT THE HARMS OF MICROPLASTICS ON THE HUMAN BODY
THE IMPACT OF COMPLAINTS ON QUALITY OF LIFE, PSYCHOLOGICAL WELL-BEING AND HEALTH OF MEDICAL WORKERS
DEVELOPMENT AND VALIDATION OF A QUESTIONNAIRE FOR PATIENTS "STUDYING THE OPINION OF PATIENTS' SATISFACTION WITH NURSE INDEPENDENT APPOINTMENT AT THE LEVEL OF PRIMARY HEALTH CARE"