Science & Healthcare

Background: Aktobe region of the Republic of Kazakhstan is a biogeochemical region of chrome and boron. There exist enough researches on the impact of various doses of chrome compounds on the human body and animals, but researches on the impact of boron compounds in the organs and systems of the body are almost absent.

Objective: to study the effects of sodium tetraborate on the morphology of the liver.

Methods: The study was conducted on 50 outbred white rats - males (10 intact, 20 control, 20 experimental). Experimental animals within 2 months daily intraperitoneally were administered sodium tetraborate 150 mg / GFP saline in a volume of 1-2 ml. Control animals at the same time were injected intraperitoneally with saline solution in a volume of 1-2 ml. The materials of the study were pieces of liver. After the slices of liver tissue processors 7-8 microns thick were stained with hematoxylin - eosin and Gieson baths. Morphometry was performed by network 40X40 square microns (250 points).

Results: In 2 months of chronic intoxication with sodium tetraborate blood stasis – filling of central vein with blood, dilation of sinusoidal capillaries, degeneration mainlyni light hepatocytes located und central vein – was being developed; the number of fibroblasts and collagen fibers around liver triade increased; amitosis of hepatocytes nuclei was noted; the increase of the number of binuclear cells (in control 6,2±0,41%, in experiment 12,7±0,75%), polyploidy cells (in control 1,2±0,02%, in experiment 4,3±0,27%). In this case there was decrease of weight index (in control 41,7±2,14%, in experiment 36,3±2,53%).

Conclusion: Chronic intoxication of a rat with sodium tetraborate results into: degeneration of hepatocytes, blood stasis in the hepatic venous vascular segments, an increase in the number of dual-core and polyploid hepatocytes.