EXPERIMENTAL ANALYSIS OF SAFETY OF ISOFLURANE LONG-TERM USE IN A CLOSED LOOP
Relevance. The main problem with the use of inhalation anesthetics in a closed loop is the interaction of halogen-containing preparations with a carbon dioxide absorber, as a result of which volatile toxic substances are released. There are several options to ensure the safety of anesthesia, one of which may be a decrease in the content of anesthetic in the gas mixture.
The purpose of the study is to experimentally test the safety hypothesis of a long-term exposure of a halogen-containing anesthetic in closed-loop mode with its low content in the respiratory mixture.
Materials and methods. Design: An experimental, controlled, prospective study. An experimental study was conducted on 40 weekly, white outbred rats, males, weighing from 190 to 220 g (average weight 201±7 g). A total of 3 series of experiments were carried out using 10 animals in each, 10 were included in the control group. In Series I, the duration of anesthesia was 4 hours, in Series II, 10 hours and in Series III, 16 hours. Inhalation anesthesia was performed using the anesthetic of isoflurane in a closed loop (carbon dioxide absorber) with an isoflurane vapor concentration in the closed loop of 0.3-0.5 vol.% And oxygen (50% in the inhaled mixture). Liver and kidney tissues were subjected to morphological analysis. The studies included determination of total protein in the blood, albumin, urea, creatinine, daily diuresis, including relative, daily excretion of protein and creatinine. Immunological studies included the determination of interleukins (TNFα, IL-2, IL-6, IL-10) and leukocytes of various differentiation clusters (common, CD3+, CD4+, CD8+, CD19+, CD95+). Parametric (Student's t-test) and non-parametric (Mann-Whitney or Wilcoxon tests) methods of statistical analysis were used.
Results. There were no significant and statistically significant differences in the main blood biochemical parameters in experimental animals during any of the examination periods, as well as changes in absolute and relative daily diuresis, increased protein excretion and changes in creatinine excretion, which completely eliminates the formation of renal failure as a result of prolonged exposure anesthetics in a closed loop.
In addition, throughout the entire study period, no signs of acute inflammatory processes or a decrease in immunological parameters were found.
Conclusion The results of the study indicate the complete safety of prolonged use of inhalation anesthesia with isoflurane in with the inclusion of carbon dioxide absorber in the circuit with the use of minimal concentrations (0.3-0.5%) of the drug in the mixture.
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Танатаров С.З. Экспериментальный анализ безопасности длительного применения изофлюрана в закрытом контуре // Наука и Здравоохранение. 2019. 2 (Т.21).С. 76-82. Tanatarov S.Z. Experimental analysis of safety of isoflurane long-term use in a closed loop // Nauka i Zdravookhranenie [Science & Healthcare]. 2019, (Vol.21) 2, pp. 76-82. Танатаров С.З. Жабық контурда изофлюранды ұзақ қолданудың қауіпсіздігін эксперименталды талдау // Ғылым және Денсаулық сақтау. 2019. 2 (Т.21). Б. 76-82.Related publications:
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