CHARACTERISTIC OF N. MENINGITIDIS STRAINS CIRCULATED IN NUR-SULTAN FROM 2010 TO 2020
Introduction. The epidemiology of meningococcal infection can change unpredictably, and the type of Neisseria meningitidis isolates play a crucial role for the surveillance of invasive meningococcus.
Purpose of the study. Conduct a molecular epidemiological study and determine the genetic characteristics of N. meningitidis strains that exist in Nur-Sultan city.
Methods. The investigation is based on the meningococcal infection registered cases in Nur-Sultan for the period 2010–2020. For characterization, Neisseria meningitidis isolates were collected from cerebrospinal fluid and blood from children. N. meningitidis isolates (n = 120) from patients were genotyped using multilocus sequence typing (MLST) and sequencing of the porB, porA, fetA, fHbp, and penA genes. Serotyping was performed using the latex agglutination method. Antimicrobial susceptibility was determined using a disk diffusion method and tested for antibiotic susceptibility using multilocus sequence typing (MLST). Statistical treatment of clinical and laboratory data was calculated using the program SPSS IBM Statistics 20. Age of different lines performed with the help of the Mann-Whitney U. test.
Results. Thus, the analysis of case histories of patients treated with a diagnosis of meningococcal meningitis in the Multidisciplinary City Children's Hospital No. 3, Nur-Sultan for 2010-2020, showed the highest proportion of detection among serogroups "A" -59.2% (n = 71), in second place "B" - 24.2% (n = 29), in third place - "C" - 10.8% (n = 13) and a low rate in serogroups "W135" -4.2% (n = 5) and "29E" - 1.7% (n = 2). The results of genome-wide analysis of N.meningitidis were deposited in the international Genbank database at the following numbers: SRX7002979, SRX7002980, SRX7002981, SRX7002982 (PubMLST, https: // www.ncbi.nlm.nih.gov / sra / term).
Conclusions. Genome-wide analysis made a significant contribution to the detailed molecular characterization of N. meningitidis isolates that were isolated in Kazakhstan over a 10-year period. Allowed to determine the level of genetic discrepancy between isolates obtained in Kazakhstan and other countries. Genetic phylogeny of complete genomes has revealed the geographical circulation of serotypes and strains, as well as cases of transmission between countries.
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Seidullayeva A.Zh., Bayesheva D.A., Turdalina B.R. , Altynbekova A.V., Turarova А.М., Daniyarov A.Zh., Kairov U.E., Kozhakhmetov S.S. Characteristic of N. Meningitidis strains circulated in Nur-Sultan from 2010 to 2020 // Nauka i Zdravookhranenie [Science & Healthcare]. 2020, (Vol.22) 6, pp. 26-34. doi 10.34689/SH.2020.22.6.004Related publications:
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