FEATURES OF IFN-γ AND IL-2 ANTIGEN-SPECIFIC PRODUCTION IN RECURRENT TUBERCULOSIS
Introduction. Tuberculosis infection is a complex interaction between mycobacterium and host resistance factors. Features of the immune response in the recurrent course of tuberculosis have not been studied enough. The article analyzes the features of antigen-specific production of interferon-γ and interleukin-2 in primary tuberculosis and relapse of the disease.
Aim: to assess the features of antigen-specific production of interferon-γ and interleukin-2 for ESAT-6, SFP-10 and TB7.7 antigens in primary and relapsed TB.
Materials and methods. A total of 208 blood samples from TB patients were analyzed. The diagnosis of tuberculosis was confirmed by molecular genetic (GeneXpert/Hain-test) or bacteriological (BACTEC) methods. There were 125 (60.1%) patients with primary tuberculosis, 83 (39.9%) patients with relapse of the disease.
Analysis of antigen-specific cytokine production was performed using standard test platforms based on cytokine release after stimulation with M. tuberculosis antigens. To compare the obtained results, standard methods of nonparametric statistics (Mann-Whitney test, median and interquartile range) were used.
Results. Comparative analysis of the production of antigen-specific IFN-γ and IL-2 showed that the level of IFN-γ was significantly increased in patients with recurrent tuberculosis compared with patients with primary tuberculosis (22±0.45 IU/ml versus 0.09±0, 21 IU/ml, respectively, p - 0.004), in the absence of differences in the level of antigen-specific IL-2 between primary and recurrent TB. At the same time, analysis of the distribution of concentrations revealed that the level of antigen-specific IFN-γ in patients with newly diagnosed TB (median ± SE = 0.03 ± 0.019 IU/ml, range 0.10–1.81 IU/ml) was significantly lower than in patients with recurrent TB (median ± SE = 0.07 ± 0.049). IU / ml, range - 0.36-3.17 IU / ml. p=0.004).
Conclusions. Antigen-specific IFN-γ and IL-2 are produced with different intensity in primary tuberculosis and relapse. The level of production of IFN-γ in primary tuberculosis is lower compared to IL-2. At the same time, there is a significant increase in IFN-γ during relapse compared with primary tuberculosis and both cytokines are synthesized with the same intensity.
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Tarabayeva A., Bitanova E., Abilbayeva A., Abubakirov A. Features of antigen-specific production of IFN-γ and IL-2 in recurrent tuberculosis // Nauka i Zdravookhranenie [Science & Healthcare]. 2022, (Vol.24) 4, pp. 79-85. doi 10.34689/SH.2022.24.4.010Related publications:
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