METAGENOMIC ANALYSIS OF GUT MICROBIAL COMMUNITIES IN KAZAKHSTAN INDIVIDUALS
Introduction. A human metagenome is 100 times larger than its own genome and determines many physiological processes in our body. The metagenome has specific characteristics for each population, which determines the markers of diseases, the course and ways of preventing and treating pathologies.
Materials and methods. The studies were carried out according to the procedures of IHMC (International Human Microbiome Consortium) standards.
Results. These studies of human metagenome are the first among the Central Asian population. Comparison of Kazakh samples of the gut microbiome with samples of other populations demonstrated the main differences and similarities and found that the microbiome depends on nutrition, climatic and geographical features, lifestyle, social factors and age.
We compared the distal gut microbiota of 149 Kazakhstan individuals aged 25 - 65 years. Our studies have shown that microbiomes are different depending on climatic and geographical features, lifestyle, social factors and age. mOTU analysis showed that a microbiome core of our population form by the genera Faecalibacterium, Bacteroides, Dorea, Collinsella, Oscillibacter, Ruminococcus, Subdoligranulum, Coprococcus, Escherichia, Eberichia, Eberichia Roseburia, Parabacteroides and Prevotella. The microbiome core does not change throughout life, and their ratio determines the human enterotype, that determine the risks of developing microbiome-associated diseases, especially the metabolism of drug substances and dietary features to maintain health. The Kazakh samples mostly belong to Enterotype 3. As well as at the mOTU level we found significant (Spearman FDR <0.05) associations to many categories of nutrients, which were studied using FFQ questionnaire. Due to study, the functionality of bacterial genes using the KEGG database were defined the 44 KEGG pathways with significant differences depending on clinical and laboratory characteristics, as well as an anamnesis.
Conclusion The main characteristics of the gut metagenome of Kazakhstan individuals were determined.
Almagul R. Kushugulova1,2*, Samat S. Kozhakhmetov1,2, Raushan Zh. Karabaeva3, Roza A. Bakenova3, Nazar K. Seidalin3, Ayaulym F. Nurgozhina1,2,
Shynggys D. Sergazy1,2, Madiyar A. Nurgaziyev1,2, Laura E. Chulenbayeva1,2, Zhanagul R. Khassenbekova2, Zhanna O. Ospanova2, Altynai K. Tuyakova1,2, Yermek O. Aitenov1,2, Alexandr E. Gulyaev1,2, Bakytgul A. Yermekbayeva4, Togzhan O. Algazina5, Gulnar R. Batpenova5, Gulnaz A. Nuranova5, Abay K. Baigenzhin6, Temirlan S. Karibekov6, Maya S. Zhumabayeva6,
Gulmira G. Dossatayeva6, Galiya M. Shaimardanova6, Larissa V. Kozina6, Talgat S. Nurgozhin7, Valery V. Benberin3, Zhaxybay Sh. Zhumadilov1,4
Institutes:
1 National Laboratory Astana Nazarbayev University,
Nur-Sultan, Republic of Kazakhstan;
2 Kazakhstan society of researchers of human microbiome”,
Nur-Sultan, Republic of Kazakhstan;
3 Medical Centre Hospital of President’s Affairs Administration of the Republic of Kazakhstan,
Nur-Sultan, Republic of Kazakhstan;
4 University Medical Center, Nazarbayev University,
Nur-Sultan, Republic of Kazakhstan;
5 Nur-sultan (Astana) Medical University,
Nur-Sultan, Republic of Kazakhstan;
6 National Scientific Medical Center",
Nur-Sultan, Republic of Kazakhstan;
7 Asfendiyarov Kazakh National Medical University,
Almaty, Republic of Kazakhstan.
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Кушугулова А.Р., Кожахметов С.С., Карабаева Р.Ж., Бакенова Р.А., Сейдалин Н.К., Нургожина А.Ф., Сергазы Ш.Д., Нургазиев М.А., Чуленбаева Л.Е., Хасенбекова Ж.Р., Оспанова Ж.О., Туякова А.К., Айтенов Е.О., Гуляев А.Е., Ермекбаева Б.А., Алгазина Т.О., Батпенова Г.Р., Нуранова Г.А., Байгенжин А.К., Карибеков Т.С., Жумабаева М.С., Досатаева Г.Г., Шаймарданова Г.М., Козина Л.В., Нургожин Т.С., Бенберин В.В., Жумадилов Ж.Ш. Метагеномный анализ кишечных бактериальных сообществ у казахстанцев // Наука и Здравоохранение. 2020. 1 (Т.22). С.48-57. doi:10.34689/SH.2020.22.1.005Похожие публикации:
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