INTERLEUKINS 10 AND 17A: THE RELATIONSHIP OF GENE POLYMORPHISMS TO DISEASE AND CYTOKINE LEVELS IN PATIENTS WITH BRUCELLOSIS IN THE KAZAKH POPULATION
Актуальность: In predicting the clinical course of brucellosis, the participation of a genetic factor, in particular, gene polymorphism of some cytokines, which are essential in the development of the disease, is not excluded. In this regard, the search for genetic markers of susceptibility and features of the course of the disease among the alleles of cytokine gene polymorphisms is of theoretical and practical interest. Aim of research was to study the associations of the polymorphisms of the IL10 and IL17 genes with brucellosis, as well as their connection with the production of the same cytokines in patients with brucellosis of Kazakh nationality. Materials and Methods. In the case-control study, there were 89 patients and 422 healthy individuals of the Kazakh population. Genotyping was performed by real-time PCR. The determination of cytokine levels in the blood plasma was carried out by enzyme immunoassay. Results. It has been established that susceptibility factors for brucellosis can be C allele and CC genotype (OR=4.42 and 7.32; 95% CI: 3.0–6.51 and 4.39–12.20, respectively), and T allele, CT, and TT genotypes of the rs8193036 polymorphism are resistance factors. Relationship was found with susceptibility to brucellosis in carriers of the G allele and GG genotype of the rs2275913 polymorphism (OR=2.26 and 2.25; 95% CI: 1.51-3.38 and 1.40-3.61), and with resistance - A allele and genotypes AA and AG. The concentration of IL-17A in patients with brucellosis with CC, CT and GG genotypes was higher compared with the control group (p=0.054, 0.002 and 0.012, respectively). Conclusions. Thus, IL17A gene polymorphisms can be associated with brucellosis disease, with the level of the cytokine itself in the Kazakh population.
Lidiya A. Mukovozova1, Nazira B. Bekenova1, Alma Z. Tokayeva1, Laura T. Kassym2, Yerbol M. Smail1 1 NJSC «Semey Medical University», Semey c., Republic of Kazakhstan; 2 Nazarbayev University, Nur-Sultan c., Republic of Kazakhstan.
1. Afzal M. S., Tahir S., Saiman A., Baig T.A., Shafi T., Zaidi N., Qadri I. Analysis of interleukin-10 gene polymorphisms and hepatitis C susceptibility in Pakistan // The Journal of Infection in Developing Countries. 2011. Vol.06. P. 473-479. 2. Ansari A., Talat N., Jamil B., Hasan Z., Razzaki T., Dawood G., Hussain R.Cytokine gene polymorphisms across tuberculosis clinical spectrum in Pakistani patients // PloS one. 2009. Vol.4 (3). P. 1-7. 3. Bazzi M. D., Sultan M. A., Al Tassan N., Alanazi M., Al-Amri A., Al-Hajjaj M. S., Warsy A.Interleukin 17A and F and Asthma in Saudi Arabia: Gene Polymorphisms and Protein Levels // Journal of Investigational Allergology and Clinical Immunology. 2011. Vol. 7. P. 551-555. 4. Bekenova N.B., Grjibovski A.M., Mukovozova L.A., Aukenov N.E. Polymorphism of IL10 gene at -1082G/A position in patients with erysipelas // Science & Healthcare. 2016. Vol. 3. P. 55-66. 5. Bekenova N.B., Grjibovski A.M., Mukovozova L.A., Smail E.M., Tokaeva A.Z. rs8193036 polymorphism of IL-17A gene in a Kazakh population and its association with plasma IL-17A among erysipelas patients. // Human Ecology. 2016. Vol. 4. P.50-55. 6. Bekenova N.B, Mukovozova L.A, Grjibovski A. M. Association of polymorphism rs 2275913 of the gene IL-17A with the level of IL-17 A in patients of Kazakh nationality with erysipelas. // Bulletin of KazNMU. 2016. Vol.1. P. 119-122. 7. Bravo M. J., de Dios Colmenero J., Alonso A., Caballero A.Polymorphisms of the interferon gamma and interleukin 10 genes in human brucellosis. //European journal of immunogenetics. 2008. Vol. 30. P.433-435. 8. Budak F., Göral G., Heper Y., Yılmaz E., Aymak F., Baştürk B., Oral H. B. IL-10 and IL-6 gene polymorphisms as potential host susceptibility factors in Brucellosis // Cytokine. 2008. Vol. 38. P. 32-36. 9. da Silva N., Germano F., Vidalez –Braz B., CarmoZanella Rd, dos Santos D., Lobato R, de Martinez A. Polymorphisms of IL-10 gene in patients infected with HCV under antiviral treatment in southern Brazil // Cytokine. 2015. Vol. 73(2). P. 253-257. 10. Du J., Han J., Li X., Zhang Y., Li H., Yang S. StIL-17 gene polymorphisms in the development of pulmonary tuberculosis // International journal of clinical and experimental pathology. 2015. Vol. 3. P. 3225-3229. 11. Gao Q. J., Liu D. W., Zhang S. Y., Jia M., Wang L. M., Wu L. H., Tong L. X.Polymorphisms of some cytokines and chronic hepatitis B and C virus infection // World J Gastroenterol. 2009. Vol. 44. P. 5610-5619. 12. Grjibovski A.The choice of statistical criterion for testing hypotheses //Human Ecology. 2008. Vol. 11. P. 48–57. 13. Grjibovski A., Ivanov S., Gorbatova M. Case-control studies in health care // Science and Health care. 2015. Vol. 4. P. 5-17. 14. Han R., Ji X., Wu B., Wang T., Han L., Yang J., Ni C. Polymorphisms in interleukin 17A gene and coal workers’ pneumoconiosis risk in a Chinese population // BMC pulmonary medicine. 2015. Vol. 1. P. 1-8. 15. Karaoglan I., Pehlivan S., Namiduru M., Pehlivan M., Kilinçarslan C., Balkan Y., Baydar I. TNF-alpha, TGF-beta, IL-10, IL-6 and IFN-gamma gene polymorphisms as risk factors for brucellosis // New Microbiology. 2009. Vol.32(2). P. 173-178. 16. Kazemi S., Saidijam M., Hashemi S. H., Karami M., Vaisi-Raygani A., Alikhani M. Y. Analysis of IL-10 and IL-6 gene polymorphisms and their serum levels in patients with brucellosis: a case control study // Immunological investigations. 2016. Vol.45(2). P. 107-115. 17. Kostina E., Molotilov B., Baranova N., Levashova O. Features of polymorphism of cytokine genes IL-4, IL-10, IL-17 A and TNF-α in patients with various clinical and pathogenetic variants of infectious-dependent bronchial asthma // Allergology and Immunology. 2013. Vol. 1. P. 5-9. 18. Li N., Zhu Q., Li Zh., Han Q., Zhang G., Chen J., Xing F., Chen Y., Zeng X., Liu J. IL17A gene polymorphisms, serum IL‐17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection // Molecular carcinogenesis. 2014. Vol. 6.P. 447-457. 19. López-Maderuelo D., Arnalich F., Serantes R., Gonzalez A., Codoceo R., Madero R., Montiel C. Interferon-γ and interleukin-10 gene polymorphisms in pulmonary tuberculosis // American journal of respiratory and critical care medicine. 2003. Vol. 7. P. 970-975. 20. Meenakshi P., Ramya S, Shruthi T, Lavanya J, Mohammed H.H., Vijayalakshmi V, Sumanlatha G. Association of IL‐1β+ 3954 C/T and IL‐10‐1082 G/A Cytokine Gene Polymorphisms with Susceptibility to Tuberculosis //Scandinavian journal of immunology. 2013. Vol. 78(1). Р. 92-97. 21. Mosaad Y., Soliman O.E, Tawhid Z.E, Sherif D.M. Interferon‐gamma+ 874 T/A and Interleukin‐10‐1082 A/G Single nucleotide Polymorphism in Egyptian Children with Tuberculosis // Scandinavian journal of immunology. 2010. Vol. 72(4). Р. 358-364. 22. Myrzabekova A. A modern characteristic of the epizootic and epidemiological situation of brucellosis in the Republic of Kazakhstan // Journal of Infectology. Application. 2015. Vol. 2. Р. 68-72. 23. Obukhov A. Overview of the Novosibirsk market of medical laboratory-diagnostic test systems // Bulletin of new medical technologies.2013. Vol. 3. P. 164-167. 24. Ocejo-Vinyals J., de Mateo E., Hoz M. The IL-17 G-152A single nucleotide polymorphism is associated with pulmonary tuberculosis in northern Spain // Cytokine. 2013. Vol.1. P. 58–61. 25. Pereira V., Sánchez-Arcila J.C, Teva A, Perce-da-Silva D.S, Vasconcelos M.P., Lima C.A., Aprígio C.J., Rodrigues-da-Silva R.N., Santos D.O., Banic D.M., Bonecini-Almeida M.G., Lima-Júnior J.C, Oliveira-Ferreira J. IL10A genotypic association with decreased IL-10 circulating levels in malaria infected individuals from endemic area of the Brazilian Amazon // Malaria journal. 2015. Vol. 28(14). P. 1-12. 26. Rafiei A., Hosseini V., Janbabai G., Ghorbani A., Ajami A., Farzmandfar T., Merrell D. S. Polymorphism in the interleukin-17A promoter contributes to gastric cancer // World J Gastroenterol. 2013. Vol. 34. P. 5693-5699. 27. Rasouli M., Asaei S., Kalani M., Kiany S., Moravej A. Interleukin-17A genetic variants can confer resistance to brucellosis in Iranian population // Cytokine. 2013. Vol. 61(1). P. 297-303. 28. Rattanasiri S., McDaniel DO, McEvoy M., Anothaisintawee T., Sobhonslidsuk A., Attia J., Thakkinstian A. The association between cytokine gene polymorphisms and graft rejection in liver transplantation: a systematic review and meta-analysis // Transplant immunology. 2013. Vol. 1. P. 62-70. 29. Rizvanova F., Pikuza O., Faizullina R., Gayfullina R., Rizvanov A., Kravtsova O. Genetic diagnostics: polymorphism of cytokine genes. Practical medicine//2010.Vol.6. P. 41-43. 30. Sepahi S., Pasdar A., Ahadi M., Gerayli S., Rostami S., Meshkat Z. Haplotype Analysis of Interleukin-10 Gene Promoter Polymorphisms in Chronic Hepatitis C Infection: A Case Control Study // Viral immunology. 2014. Vol. 27(8). P. 398-403. 31. Simbirtsev A., Gromova A. Functional polymorphism of the genes of regulatory molecules of inflammation // Cytokines and inflammation. 2005. Vol. 1. P.1-10. 32. Stappers M., Thys Y., Oosting M., Plantinga T., Ioana M., Reimnitz P., Mouton J., Netea M., Joosten L. Polymorphisms in cytokine genes IL6, TNF, IL10, IL17A and IFNG influence susceptibility to complicated skin and skin structure infections // European Journal of Clinical Microbiology & Infectious Diseases. 2014. Vol. 33(12). P. 2267-2274. 33. Subbotina A., Grjibovski A. Descriptive statistics and verification of the normality of quantitative data distribution // Human ecology. 2014. Vol. 2. P.51-57. 34. Ungureanu T., Grjibovski A. Software for statistical data processing STATA: introduction // Human ecology. 2014. Vol. 1. P. 60-63. 35. Wang J., Liu Y., Xie L., Li S., Qin X. Interleukin-10 promoter polymorphisms in patients with hepatitis B virus infection or hepatocellular carcinomain Chinese Han ethnic population // HepatobiliaryPancreat Dis Int. 2005. Vol. 1. P.60-64. 36. Wang J., Shyur S., Wang W., Liou Y., Lin C., Wu Y., Wu L. The polymorphisms of interleukin 17A (IL17A) gene and its association with pediatric asthma in Taiwanese population // Allergy. 2009.Vol. 64(7). P.1056-1060. 37. Wang L., Jiang Y., Zhang Y., Wang Y., Huang S., Wang Z., Pang D. Association analysis of IL-17A and IL-17F polymorphisms in Chinese Han women with breast cancer // PloS one. 2012. Vol.7 (3). P.1-7. 38. Zhandosov Sh.U. Analysis of the epidemiological situation of brucellosis in the Republic of Kazakhstan for 2008-2010. Materials of the International Scientific and Practical Conference "Zoonotic infections: yesterday, today, tomorrow", Almaty, Kazakhstan. December 24-25. 2011; 7-9. 39. Zhang T., Pan F. M, Zhang L. Z,Gao Y. F, Zhang Z. H, Gao J, Ge R, Mei Y, Shen B. B, Duan Z. H, Li X. A meta-analysis of the relation of polymorphism at sites− 1082 and− 592 of the IL-10 gene promoter with susceptibility and clearance to persistent hepatitis B virus infection in the Chinese population // Infection. 2011. Vol. 39(1). P. 21-27.
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Mukovozova L.A., Bekenova N.B., Tokayeva A.Z., Kassym L.T., Smail E.M. Interleukins 10 and 17A: the relationship of gene polymorphisms to disease and cytokine levels in patients with brucellosis in the Kazakh population // Nauka i Zdravookhranenie [Science & Healthcare]. 2022, (Vol.24) 1, pp. 39-46. doi 10.34689/SH.2022.24.1.005