CHANGES OF PLASMA FREE AMINO ACIDS IN METABOLIC SYNDROME
Background: Studies have been going on for a while now to investigate the link between plasma free amino acids (PFAA) and metabolic syndrome, and the results so far have been quite promising. However, these analyses aren't commonly used for early clinical diagnosis of metabolic syndrome, partly because mass spectrometry can be pretty expensive. That's why we decided to look into and characterize the PFAA profile of blood plasma in metabolic syndrome patients using a more affordable alternative to MS\MS or GC-MS methods: high performance liquid chromatography (HPLC).
Objective: How can we describe the changes in the levels of free amino acids found in the bloodstream of individuals diagnosed with metabolic syndrome using high-performance liquid chromatography (HPLC)?
Materials and methods: We sampled thirty patients who met the diagnostic criteria for metabolic syndrome from their plasma for PFAA determination using HPLC. Additionally, we took plasma samples from fifteen confirmed healthy individuals and analyzed them using HPLC. We measured the concentrations in the samples and analyzed the relationship between the profiles of free amino acids in the plasma.
Results and discussion: The results show a significant difference in plasma concentrations of alanine, cysteine, tyrosine, valine, cystine and isoleucine between the two groups. The HPLC method, when utilized under specific conditions, can be employed for the quantitative measurement of amino acids in the blood.
Alma Nurtazina1, https://orcid.org/0000-0002-9737-4003
Ivan Voitsekhovskiy2, https://orcid.org/0000-0001-6920-2776
Maxat Toishimanov3, https://orcid.org/0000-0002-6070-4574
Daulet Dautov4, https://orcid.org/0000-0003-0838-6983
1 NJSC «Semey medical university», Semey, Republic of Kazakhstan;
2 Al Farabi Kazakh National University, Almaty, Republic of Kazakhstan;
3 The Kazakhstan-Japan Innovation Center KazNARU, Almaty, Republic of Kazakhstan;
4 NJSC “Asfendiyarov Kazakh National Medical University”, Almaty, Republic of Kazakhstan.
1. Saklayen M.G. The global epidemic of the metabolic syndrome // Current hypertension reports. 2018 Feb. 20(2):1-8.
2. GBD 2015 Obesity Collaborators. Health effects of overweight and obesity in 195 countries over 25 years // New England journal of medicine. 2017 Jul 6;377(1):13-27.
3. Malik V.S., Hu F.B. Sugar-sweetened beverages and cardiometabolic health: an update of the evidence // Nutrients. 2019 Aug 8. 11(8):1840.
4. Nurtazina A., Kozhakhmetova D., Dautov D., Shakhanova A., Chattu V.K. Apolipoprotein B/A1 ratio as a diagnostic alternative to triglycerides and HDL-Cholesterol for the prediction of metabolic syndrome among hypertensives in Kazakhstan // Diagnostics. 2020, Jul 23. 10(8):510.
5. Giesbertz P., Daniel H. Branched-chain amino acids as biomarkers in diabetes // Current Opinion in Clinical Nutrition & Metabolic Care. 2016 Jan 1. 19(1):48-54.
6. Zhenyukh O., Civantos E., Ruiz-Ortega M., Sánchez M.S., Vazquez C., Peiro C., Egido J., Mas S. High concentration of branched-chain amino acids promotes oxidative stress, inflammation and migration of human peripheral blood mononuclear cells via mTORC1 activation // Free Radical Biology and Medicine. 2017 Mar 1. 104:165-77.
7. Wiklund P.K., Pekkala S., Autio R., Munukka E., Xu L., Saltevo J., Cheng S., Kujala U.M., Alen M., Cheng S. Serum metabolic profiles in overweight and obese women with and without metabolic syndrome // Diabetology & metabolic syndrome. 2014 Dec. 6(1):1-9.
8. Alberti K.G. International diabetes federation task force on epidemiology and prevention; national heart, lung, and blood institute; American heart association; world heart federation; international atherosclerosis society; international association for the study of obesity: harmonizing the metabolic syndrome: a joint interim statement of the international diabetes federation task force on epidemiology and prevention; national heart, lung, and blood institute; American heart association; world heart federation // Circulation. 2009. 120:1640-5.
9. Yamaguchi N., Mahbub M.H., Takahashi H., Hase R,. Ishimaru Y., Sunagawa H., Amano H., Kobayashi-Miura M., Kanda H. et al. Plasma free amino acid profiles evaluate risk of metabolic syndrome, diabetes, dyslipidemia, and hypertension in a large Asian population // Environmental health and preventive medicine. 2017 Dec. 22:1-8.
10. Sun S., He D., Luo C., Lin X., Wu J., Yin X., et al. Metabolic Syndrome and Its Components Are Associated with Altered Amino Acid Profile in Chinese Han Population // Frontiers in endocrinology. 2022 Jan 4. 12:795044.
11. Ikeda H. The Effect of Mild Renal Dysfunction on the Assessment of Plasma Amino Acid Concentration and Insulin Resistance in Patients with Type 2 Diabetes Mellitus // Journal of Diabetes Research. 2022 Jun 13.
12. Bagheri M., Djazayery A., Farzadfar F., Qi L., Yekaninejad M.S., Aslibekyan S., Chamari M., Hassani H., Koletzko B., Uhl O. Plasma metabolomic profiling of amino acids and polar lipids in Iranian obese adults // Lipids in health and disease. 2019 Dec. 18(1):1-9.
13. Wewer Albrechtsen N.J., Junker A.E., Christensen M., Hædersdal S., Wibrand F., Lund A.M., Galsgaard K.D., Holst J.J., Knop F.K., Vilsbøll T. Hyperglucagonemia correlates with plasma levels of non-branched-chain amino acids in patients with liver disease independent of type 2 diabetes // American Journal of Physiology-Gastrointestinal and Liver Physiology. 2018 Jan 1;314(1):G91-6.
14. Remchak M.M., Heiston E.M., Ballantyne A., Dotson B.L., Stewart N.R., Spaeth A.M., Malin S.K. Insulin sensitivity and metabolic flexibility parallel plasma TCA levels in early chronotype with metabolic syndrome // The Journal of Clinical Endocrinology & Metabolism. 2022 Jul 14;107(8):e3487-96.
15. Kaur P., Rizk N., Ibrahim S., Luo Y., Younes N., Perry B., Dennis K., Zirie M., Luta G., Cheema A.K. Quantitative metabolomic and lipidomic profiling reveals aberrant amino acid metabolism in type 2 diabetes // Molecular BioSystems. 2013. 9(2):307-17.
16. Zhong F., Xu M., Bruno R.S., Ballard K.D., Zhu J. Targeted high performance liquid chromatography tandem mass spectrometry-based metabolomics differentiates metabolic syndrome from obesity. Experimental Biology and Medicine. 2017 Apr;242(7):773-80.
17. Lu J., Xie G., Jia W., Jia W. Insulin resistance and the metabolism of branched-chain amino acids // Frontiers of medicine. 2013 Mar;7:53-9.
Количество просмотров: 539
Категория статей:
Оригинальные исследования
Библиографическая ссылка
Nurtazina A., Voitsekhovskiy I., Toishimanov M., Dautov D. Сhanges of plasma free amino acids in metabolic syndrome // Nauka i Zdravookhranenie [Science & Healthcare]. 2023, (Vol.25) 2, pp. 84-89. doi 10.34689/SH.2023.25.2.012Похожие публикации:
TRENDS IN MORBIDITY AND MORTALITY FROM CHRONIC HEART FAILURE IN ALMATY
СТРУКТУРНО-ФУНКЦИОНАЛЬНЫЕ ИЗМЕНЕНИЯ МИОКАРДА ПОСЛЕ АОРТОКОРОНАРНОГО ШУНТИРОВАНИЯ У ПАЦИЕНТОВ С ИЗБЫТОЧНОЙ МАССОЙ ТЕЛА НА РАЗНЫХ ЭТАПАХ РЕАБИЛИТАЦИИ
МЕДИКО – СОЦИАЛЬНЫЕ, ПСИХОЛОГИЧЕСКИЕ АСПЕКТЫ КАЧЕСТВА ЖИЗНИ БОЛЬНЫХ С ОСТРЫМ ПАНКРЕАТИТОМ
A COMPARATIVE ANALYSIS OF 24-HOUR DIETARY RECALLS AND FOOD FREQUENCY QUESTIONNAIRES ADMINISTERED SIMULTANEOUSLY IN THE KAZAKHSTANI POPULATION
ANALYSIS OF PATIENT SATISFACTION WITH THE QUALITY OF ORGANIZATION OF TREATMENT IN FOREIGN CLINICS