RADIATION-INDUCED KI-67 PROLIFERATION IN THE SMALL INTESTINE OF RATS
Introduction. It is known that Hiroshima and Nagasaki were target of the first atomic bombs in the history of mankind. As a result, the population of Japanese cities was exposed to internal and external irradiation. Numerous authors maintain that primary neutron-activated radionuclides are chemical elements 56Mn and 60Co. Herewith, 56Mn was determined the dominant role of neutrons induced by β-radiation during the first few hours after the atomic bombing. The systemic access in estimation of Кi-67 marker in radiosensitive small intestine defining the functional state and the interrelation of structural components serves as a combination of diagnostic criterion and prognosis.
The objective of study. To assess the prognostic significance of Ki-67 antigen for detection of cell proliferative activity in the small intestine of rats exposed to low dose of β− and γ–radiation.
Materials and methods. In experiment that held in February 2016, the male sex «Wistar» rats at 5 month old and in amount of 90, weighting approximately 270−350 g. Three groups were identified: 1) 56Mn which obtained by neutron activation of 100 mg MnO2 powder using the «Baikal−1» nuclear reactor with a neutrons fluence of 4×1014 n/cm²; 2) 60Co γ−rays; 3) control group. Necropsy of the animals were on the 3rd, 14th and 60th days after irradiation, then the small intestine removed, after which it was fixed in 10% formalin. Paraffin sections were dewaxed and rehydrated using the method of D.Sarkisov and Yu.Perov (1996). To visualize the immunohistochemical reaction, the DAB+(DAKO) system was used. The number of Ki-67-positive cells, taking into account the colored nuclei of any intensity, expressing the results in percent. All the presented data and results were expressed as mean (M), median (Me) and interquartile interval (IQR). Statistical comparisons were made by Kruskel-Wallis test (SPSS 2,0). A p<0,05 was considered statistically significant.
Results. When comparing microscopic processes occurring in the rats small intestinal tissue after exposure to neutron-activated manganese dioxide and external irradiation, the most pronounced morphofunctional disorders which detected by the number of Ki-67-positive cells are noted in the later periods after 56Mn effect. The studied parameters of the small intestine have statistically significant differences in the control and irradiated animal groups (p<0,001).
Conclusion. Morphofunctional disorders in the small intestine that result from internal and external irradiation are characterized by a change in the immunohistochemical indicator of Ki-67, indicative of cell proliferation in late terms. Comparing the level of Ki-67 antigen in the organ of various animal groups studied, a high level was observed in the late periods after exposure to neutron-activated manganese dioxide.
Количество просмотров: 2390
Категория статей:
Оригинальные исследования
Библиографическая ссылка
Узбeкoв Д.E., Шaбдapбaeвa Д.М., Чaйжyнycoвa Н.Ж., Caякeнoв Н.Б., Узбeкoвa C.E., Aмaнтaeвa Г.K., Pycлaнoвa Б., Аубакирова Г.T., Aбeyoвa A.C., Xoши M., Шалгумбаева Г.М. Paдиaциoннo-индуциpoвaннaя пpoлифepaция КI-67 в тoнкoй кишкe кpыc // Наука и Здравоохранение. 2019. 1 (Т.21). С. 63-73. Uzbekov D.E., Shabdarbaeva D.M., Chaizhunusova N.Zh., Sayakenov N.B., Uzbekova S.E., Amantaeva G.K., Ruslanova B., Aubakirova G.T., Abeuova A.S., Hoshi M., Shalgumbayeva G.M. Radiation-induced KI-67 proliferation in the small intestine of rats. Nauka i Zdravookhranenie [Science & Healthcare]. 2019, (Vol.21) 1, pp. 63-73. Узбeкoв Д.E., Шaбдapбaeвa Д.М., Чaйжyнycoвa Н.Ж., Caякeнoв Н.Б., Узбeкoвa C.E., Aмaнтaeвa Г.K., Pycлaнoвa Б., Аубакирова Г.T., Aбeyoвa A.C., Xoши M., Шалгумбаева Г.М. Paдиaция әcepiнeн туындaғaн eгeуқұйpықтap жiңiшкe iшeгiндeгi КI-67 пpoлифepaцияcы // Ғылым және Денсаулық сақтау. 2019. 1 (Т.21). Б. 63-73.Похожие публикации:
TRENDS IN MORBIDITY AND MORTALITY FROM CHRONIC HEART FAILURE IN ALMATY
СТРУКТУРНО-ФУНКЦИОНАЛЬНЫЕ ИЗМЕНЕНИЯ МИОКАРДА ПОСЛЕ АОРТОКОРОНАРНОГО ШУНТИРОВАНИЯ У ПАЦИЕНТОВ С ИЗБЫТОЧНОЙ МАССОЙ ТЕЛА НА РАЗНЫХ ЭТАПАХ РЕАБИЛИТАЦИИ
МЕДИКО – СОЦИАЛЬНЫЕ, ПСИХОЛОГИЧЕСКИЕ АСПЕКТЫ КАЧЕСТВА ЖИЗНИ БОЛЬНЫХ С ОСТРЫМ ПАНКРЕАТИТОМ
A COMPARATIVE ANALYSIS OF 24-HOUR DIETARY RECALLS AND FOOD FREQUENCY QUESTIONNAIRES ADMINISTERED SIMULTANEOUSLY IN THE KAZAKHSTANI POPULATION
ANALYSIS OF PATIENT SATISFACTION WITH THE QUALITY OF ORGANIZATION OF TREATMENT IN FOREIGN CLINICS