Online ISSN: 3007-0244,
Print ISSN:  2410-4280
INTERLEUKINS 10 AND 17A: THE RELATIONSHIP OF GENE POLYMORPHISMS TO DISEASE AND CYTOKINE LEVELS IN PATIENTS WITH BRUCELLOSIS IN THE KAZAKH POPULATION
Актуальность: In predicting the clinical course of brucellosis, the participation of a genetic factor, in particular, gene polymorphism of some cytokines, which are essential in the development of the disease, is not excluded. In this regard, the search for genetic markers of susceptibility and features of the course of the disease among the alleles of cytokine gene polymorphisms is of theoretical and practical interest. Aim of research was to study the associations of the polymorphisms of the IL10 and IL17 genes with brucellosis, as well as their connection with the production of the same cytokines in patients with brucellosis of Kazakh nationality. Materials and Methods. In the case-control study, there were 89 patients and 422 healthy individuals of the Kazakh population. Genotyping was performed by real-time PCR. The determination of cytokine levels in the blood plasma was carried out by enzyme immunoassay. Results. It has been established that susceptibility factors for brucellosis can be C allele and CC genotype (OR=4.42 and 7.32; 95% CI: 3.0–6.51 and 4.39–12.20, respectively), and T allele, CT, and TT genotypes of the rs8193036 polymorphism are resistance factors. Relationship was found with susceptibility to brucellosis in carriers of the G allele and GG genotype of the rs2275913 polymorphism (OR=2.26 and 2.25; 95% CI: 1.51-3.38 and 1.40-3.61), and with resistance - A allele and genotypes AA and AG. The concentration of IL-17A in patients with brucellosis with CC, CT and GG genotypes was higher compared with the control group (p=0.054, 0.002 and 0.012, respectively). Conclusions. Thus, IL17A gene polymorphisms can be associated with brucellosis disease, with the level of the cytokine itself in the Kazakh population.
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Mukovozova L.A., Bekenova N.B., Tokayeva A.Z., Kassym L.T., Smail E.M. Interleukins 10 and 17A: the relationship of gene polymorphisms to disease and cytokine levels in patients with brucellosis in the Kazakh population // Nauka i Zdravookhranenie [Science & Healthcare]. 2022, (Vol.24) 1, pp. 39-46. doi 10.34689/SH.2022.24.1.005

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