COMPARATIVE EVALUATION OF BLOOD COLLECTION TUBES AND EXTRACTION METHODS FOR CIRCULATING TUMOR DNA
Introduction. Circulating tumor DNA (ctDNA) has emerged as a valuable biomarker for non-invasive cancer diagnostics and treatment monitoring. However, pre-analytical variables, including blood collection and sample processing, significantly influence ctDNA yield and analytical reliability. Optimizing these conditions is crucial for ensuring reproducible and accurate ctDNA measurements.
Aim. To perform a comparative evaluation of blood collection tubes and DNA extraction methods for the isolation of circulating tumor DNA from the blood plasma of patients with oncological diseases.
Materials and Methods. Plasma samples were collected using two types of blood collection tubes: PaxGene and Streck. ctDNA was extracted using three different systems: Raissol, MagMax, and QiaAmp. Quantitative performance, reproducibility, and variability of ctDNA yield were assessed across all combinations of collection tubes and extraction methods. Statistical analysis was performed to evaluate differences among extraction systems.
Discussion. The results demonstrated that QiaAmp consistently provided higher ctDNA concentrations across both collection systems. Samples collected in PaxGene tubes exhibited lower variability and improved analytical stability compared with Streck tubes. Statistically significant differences among extraction methods were observed for PaxGene samples, while increased variability in Streck samples limited the ability to discriminate between methods. These findings emphasize the critical role of pre-analytical optimization in ctDNA workflows and support the implementation of standardized protocols to improve reliability in liquid biopsy analysis.
Conclusion. The results demonstrate that both blood collection tubes and the DNA extraction method significantly affect ctDNA recovery, highlighting the importance of optimizing and standardizing pre-analytical workflows for reliable liquid biopsy analysis.
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Aitkulova A.M., Kadenova T.B., Gabdulkayum A.K., Yerezhepov D.A., Rakhimova S.Ye., Kozhamkulov U.A., Kairov U.E., Yermekbayeva B.A., Akilzhanova A.R., Sarbassov D.D. Comparative evaluation of blood collection tubes and extraction methods for circulating tumor DNA isolation // Nauka i Zdravookhranenie [Science & Healthcare]. 2025. Vol.27 (6), pp. 7-13. doi 10.34689/SH.2025.27.6.001Ұқсас жариялымдар:
COMPARATIVE EVALUATION OF BLOOD COLLECTION TUBES AND EXTRACTION METHODS FOR CIRCULATING TUMOR DNA
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